School of Engineering and Mathematical Sciences, City University London, Northampton Square, London, EC1V 0HB, UK.
J Mol Recognit. 2012 Jan;25(1):1-10. doi: 10.1002/jmr.1163.
Although N-isopropylacrylamide (NIPAM) has previously been used in molecular imprinting, it has mostly been considered as an 'inert' monomer, or included for its temperature-responsive nature, rather than as a functional monomer responsible for the interactions with the template at the recognition site. A comparative study of NIPAM and other traditional, functional monomers for the imprinting of a hydrogen bond donor template, bisphenol A (BPA), is reported. Nuclear magnetic resonance titration data suggest that NIPAM forms a stronger complex with BPA than either acrylamide or methacrylic acid but a weaker complex than vinylpyridine. Molecular imprinted polymers (MIPs) were prepared using each functional monomer and compared as stationary phases for the separation of BPA from structural analogues. The NIPAM-containing MIP bound BPA with better selectivity than those prepared using acrylamide or methacrylic acid. Using NIPAM also reduces the nonspecific binding, which is found with MIPs using vinylpyridine as functional monomer.
虽然 N-异丙基丙烯酰胺（NIPAM）以前曾用于分子印迹，但它主要被认为是一种“惰性”单体，或者因其温度响应特性而被包含，而不是作为负责在识别位点与模板相互作用的功能单体。本文报道了 NIPAM 与其他传统功能单体在氢键供体模板双酚 A（BPA）印迹方面的比较研究。核磁共振滴定数据表明，NIPAM 与 BPA 形成的复合物比丙烯酰胺或甲基丙烯酸强，但比乙烯基吡啶弱。使用每种功能单体制备了分子印迹聚合物（MIP），并将其作为固定相进行比较，以从结构类似物中分离 BPA。含 NIPAM 的 MIP 对 BPA 的选择性结合优于使用丙烯酰胺或甲基丙烯酸制备的 MIP。使用 NIPAM 还可以减少使用乙烯基吡啶作为功能单体的 MIP 中的非特异性结合。
