Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya 663-8179, Japan.
Talanta. 2010 Mar 15;80(5):1713-8. doi: 10.1016/j.talanta.2009.10.011. Epub 2009 Oct 14.
Three monodispersed, molecularly imprinted polymers (MIPs) for cinchonidine (CD) have been synthesized by precipitation polymerization. MIP1 was prepared using methacrylic acid (MAA) as a functional monomer and divinylbenzene (DVB) as a cross-linker and MIP2 was prepared with further addition of 2-hydroxyethyl methacrylate (HEMA) as a co-monomer. For the preparation of MIP3, core-shell type MIP, monodispersed DVB homopolymers, which are prepared by precipitation polymerization, were used as a core and CD-imprinted MAA-DVB copolymer phases were coated onto the core. Three MIPs synthesized gave monodispersed, spherical beads in micrometer sizes. The binding characteristics and molecular recognition properties of MIP1-3 were examined by Scatchard analysis and chromatographic studies. The association constant of CD with MIP1 was the highest among MIPs prepared, while that with MIP3 was the lowest. The template molecule, CD, was more retained than its stereoisomer, cinchonine, on the three MIPs, and the stereoseparation factor of 38 was obtained with MIP3.
三种单分散的、分子印迹聚合物（MIPs）已经通过沉淀聚合合成了，用于金鸡纳碱（CD）。MIP1 是用甲基丙烯酸（MAA）作为功能单体和二乙烯基苯（DVB）作为交联剂合成的，而 MIP2 是进一步添加 2-羟乙基甲基丙烯酸酯（HEMA）作为共单体合成的。为了制备 MIP3，核壳型 MIP，即通过沉淀聚合制备的单分散 DVB 均聚物，被用作核，然后将 CD 印迹的 MAA-DVB 共聚物相涂覆在核上。三种合成的 MIP 均得到了微米级的单分散球形珠粒。通过 Scatchard 分析和色谱研究考察了 MIP1-3 的结合特性和分子识别性能。MIP1 与 CD 的结合常数在所有制备的 MIP 中是最高的，而 MIP3 与 CD 的结合常数是最低的。模板分子 CD 在三种 MIP 上的保留性均高于其对映异构体金鸡纳碱，并且 MIP3 获得了 38 的立体分离因子。
