调控因子X1基因的敲除导致早期胚胎致死。
Knockout of the regulatory factor X1 gene leads to early embryonic lethality.
作者信息
Feng Chenzhuo, Xu Wenhao, Zuo Zhiyi
机构信息
Department of Anesthesiology, School of Medicine, University of Virginia, Charlottesville, VA, USA.
出版信息
Biochem Biophys Res Commun. 2009 Sep 4;386(4):715-7. doi: 10.1016/j.bbrc.2009.06.111. Epub 2009 Jun 25.
Abstract
The biological function of regulatory factor X1 (RFX1), the prototype member of the transcription factor RFX family, is not clear. We have used gene trap technique to disrupt the expression of RFX1 in mice. Although, heterozygous RFX1(+/-) mice appear normal and fertile, homozygous RFX1(-/-) embryos died at an early stage (most likely before embryonic day 2.5). Our results indicate that RFX1 regulates expression of genes that are essential for early embryonic development/survival and that RFX1 function can not be compensated by other RFX1 family members.
摘要
转录因子RFX家族的原型成员调控因子X1（RFX1）的生物学功能尚不清楚。我们利用基因捕获技术破坏了小鼠中RFX1的表达。虽然杂合子RFX1（+/-）小鼠看起来正常且可育，但纯合子RFX1（-/-）胚胎在早期死亡（很可能在胚胎第2.5天之前）。我们的结果表明，RFX1调节早期胚胎发育/存活所必需的基因的表达，并且RFX1的功能不能被其他RFX1家族成员补偿。
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