用于流式细胞术分析酿酒酵母孢子形成的荧光DNA结合染料的比较
A comparison of fluorescent DNA binding dyes for flow cytometric analysis of sporulating Saccharomyces cerevisiae.
作者信息
Raithatha Sheetal A, Stuart David T
机构信息
University of Alberta, Department of Biochemistry, 561 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H6.
出版信息
J Microbiol Methods. 2009 Sep;78(3):357-9. doi: 10.1016/j.mimet.2009.06.015. Epub 2009 Jun 25.
PMID:19559735
Abstract
Flow cytometry is important tool for investigating DNA replication in sporulating Saccharomyces cerevisiae. However, flow cytometry data from maturing spores is often difficult to interpret due to extensive broadening of the fluorescence peaks. This problem is markedly improved by treatment of the spores with potassium hydroxide prior to staining.
摘要
流式细胞术是研究酿酒酵母孢子形成过程中DNA复制的重要工具。然而，由于荧光峰的广泛展宽，成熟孢子的流式细胞术数据往往难以解释。在染色前用氢氧化钾处理孢子可显著改善这一问题。
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