以胶体金为载体生产抗血小板活化因子抗体。
Production of anti-platelet-activating factor antibodies by the use of colloidal gold as carrier.
作者信息
Tomii A, Masugi F
机构信息
Department of Medicine and Geriatrics, Osaka University Medical School, Japan.
出版信息
Jpn J Med Sci Biol. 1991 Apr;44(2):75-80. doi: 10.7883/yoken1952.44.75.
Polyclonal and monoclonal antibodies to platelet-activating factor (PAF) were produced, by use of colloidal gold as the hapten carrier. These polyclonal (R88-09) and monoclonal (602 B11) antibodies both reacted with PAF, lysoplatelet-activating factor (lyso PAF), and L-alpha-lysophosphatidylcholine, palmitoyl (lyso PCP), but did not react with phosphorylcholine chloride (PCC). Their affinities were higher for PAF than lyso PAF and lyso PCP.
利用胶体金作为半抗原载体，制备了针对血小板活化因子（PAF）的多克隆抗体和单克隆抗体。这些多克隆抗体（R88 - 09）和单克隆抗体（602 B11）均能与PAF、溶血血小板活化因子（lyso PAF）、L-α-溶血磷脂酰胆碱、棕榈酰（lyso PCP）发生反应，但不与氯化磷酰胆碱（PCC）反应。它们对PAF的亲和力高于lyso PAF和lyso PCP。
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