重组酶介导的盒式交换为基因靶向提供了一个通用的平台：miR-31b 的敲除。
Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: knockout of miR-31b.
机构信息
Temasek Life Sciences Laboratory, National University of Singapore, 117604, Republic of Singapore.
出版信息
Genetics. 2009 Sep;183(1):399-402. doi: 10.1534/genetics.109.105213. Epub 2009 Jun 29.
Abstract
A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described.
摘要
已经设计了一系列载体来增强靶向同源重组的多功能性。重组酶介导的盒式交换允许在任何基因座进行顺序靶向，并提高了用户定义突变的灵活性。描述了 RMCE 在删除内含子 microRNA 基因中的应用。
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