Department of Botany, Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04167 Košice, Slovak Republic.
Environ Toxicol. 2010 Dec;25(6):581-92. doi: 10.1002/tox.20519.
Tolerance of three Slovak populations of Silene vulgaris [from the localities "Harmanec" (total soil Cu 34.1 mg kg⁻¹), "Lubietová"; (total soil Cu 831.6 mg kg⁻¹) and "Špania dolina" (total soil Cu 1368.7 mg kg⁻¹) referred as SV1, SV2, and SV3, respectively] and Silene dioica (SD; from the locality "Špania dolina") exposed to 60 μM Cu for 7 days has been studied. Respective controls with 0.21 μM Cu were also cultured. SV3 and SD contained higher total shoot Cu than SV1. Methanol-soluble shoot Cu represented 60, 59, 59, and 55% from total shoot Cu and roots contained 16.7-, 20.4-, 9.9- and 28.9-fold more Cu than shoots in SV1, SV2, SV3, and SD, respectively. Soluble proteins were the least affected in SV3 and SD when Cu-exposed and control plants were compared. Root hydrogen peroxide and superoxide showed similar trend and were correlated with enhancement of ascorbate- and guaiacol-peroxidase activities. Malondialdehyde accumulation increased in SV1 and SV2. Phenylalanine ammonia-lyase activity and total soluble phenols were higher in SV3 Cu-exposed plants compared to SV1 and SV2. Shikimate dehydrogenase activity was enhanced in all Cu-exposed populations. Cinnamyl alcohol dehydrogenase activity and root lignin content were not affected and polyphenol oxidase activity was not detected. Within 14 detected free amino acids, majority of them decreased preferentially in the roots. Only serine increased in shoots and decreased in roots of all populations. Potassium content was not affected in SV3. Na, Ca, and Fe increased in roots of Cu-exposed plants of all populations, while Mg was the least affected and Zn increased in SV2 and SV3 shoots and roots. This study revealed the highest tolerance in population from the locality with the highest soil Cu content. Present findings as complex metabolic responses to Cu stress with special emphasis on phenolic metabolism are discussed.
已经研究了三种斯洛伐克矢车菊种群[分别来自“Harmanec”（总土壤铜 34.1mg/kg）、“Lubietová”（总土壤铜 831.6mg/kg）和“Špania dolina”（总土壤铜 1368.7mg/kg），分别称为 SV1、SV2 和 SV3]和琉璃苣（SD；来自“Špania dolina”）在暴露于 60μM Cu 7 天后的耐受性。还培养了相应的 0.21μM Cu 对照。SV3 和 SD 的总茎 Cu 含量高于 SV1。甲醇可溶茎 Cu 占总茎 Cu 的 60、59、59 和 55%，而根 Cu 含量分别比 SV1、SV2、SV3 和 SD 中的茎 Cu 高 16.7、20.4、9.9 和 28.9 倍。与对照植物相比，Cu 暴露时 SV3 和 SD 中的可溶性蛋白受影响最小。根过氧化氢和超氧化物表现出相似的趋势，与增强抗坏血酸和愈创木酚过氧化物酶活性相关。丙二醛积累在 SV1 和 SV2 中增加。与 SV1 和 SV2 相比，SV3 Cu 暴露植物中的苯丙氨酸解氨酶活性和总可溶性酚含量较高。所有 Cu 暴露种群的莽草酸脱氢酶活性均增强。肉桂醇脱氢酶活性和根木质素含量不受影响，多酚氧化酶活性未检测到。在 14 种检测到的游离氨基酸中，大多数优先在根中减少。只有丝氨酸在所有种群的茎中增加，在根中减少。SV3 中的钾含量不受影响。Na、Ca 和 Fe 在所有 Cu 暴露植物的根中增加，而 Mg 受影响最小，Zn 在 SV2 和 SV3 的茎和根中增加。本研究揭示了来自土壤 Cu 含量最高的地点的种群具有最高的耐受性。讨论了对 Cu 胁迫的这种复杂代谢反应，特别强调了酚类代谢。
