土茯苓复方中药对小鼠铅中毒的治疗作用

[Therapeutic effects of traditional Chinese herbal formula with Smilax glabra on mice's lead poisoning].

作者信息

Xia Dao-Zong, Wang Hui-Ming, Yang Qing, Jiang Li-Qin, Xia Ming

机构信息

College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.

出版信息

Zhong Yao Cai. 2009 Mar;32(3):404-6.

PMID:19565723

Abstract

OBJECTIVE

To investigate the influence of traditional Chinese herbal formula with Smilax glabra on lead expelling and oxidative damage in lead-poisoned mice.

METHODS

Mice were received intraperitoneal injections of lead acetate to establish lead-poisoned models. The concentration of lead, zinc and copper in blood, brain, liver, kidney and bone were determined. The lipid peroxidation of liver was systematically evaluated.

RESULTS

The traditional Chinese herbal formula had no influence on body weight and organ weight/body weight ratio of lead-poisoned mice. The traditional Chinese herbal formula might significantly reduce the concentration of lead in blood, rain, liver, kidney and bone, and the rate were 74%, 48%, 56%, 51% and 44%, but had no influence on zinc's and copper' s concentration. The decreasing rate of MDA levels in liver by the traditional Chinese herbal formula was 31%. In addition, the increase rate of GSH levels and the activities of GSH-Px and SOD in liver by traditional Chinese herbal formula were 63% ,76% and 66%, respectively.

CONCLUSIONS

The traditional Chinese herbal formula with Smilax glabra can significantly advance the excretion of lead and protect liver against oxidative damage induced by lead.

摘要

目的

探讨土茯苓复方中药对铅中毒小鼠排铅及氧化损伤的影响。

方法

给小鼠腹腔注射醋酸铅建立铅中毒模型，测定血、脑、肝、肾及骨中铅、锌、铜的含量，并系统评价肝脏脂质过氧化情况。

结果

土茯苓复方中药对铅中毒小鼠体重及脏器重量/体重比值无影响。可显著降低血、脑、肝、肾及骨中铅含量，降低率分别为74%、48%、56%、51%和44%，对锌、铜含量无影响。肝脏丙二醛（MDA）含量降低率为31%。此外，肝脏谷胱甘肽（GSH）含量升高率、谷胱甘肽过氧化物酶（GSH-Px）及超氧化物歧化酶（SOD）活性升高率分别为63%、76%和66%。