College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Binjiang District, Hangzhou, China.
J Ethnopharmacol. 2010 Jul 20;130(2):414-20. doi: 10.1016/j.jep.2010.05.025. Epub 2010 May 24.
Smilax glabra Roxb. is a traditional Chinese herb, the rhizome of Smilax glabra has been used in folk medicine for the treatment of lead poisoning.
The present study was conducted to investigate the protective role of Smilax glabra extract (SGE) individually or combined with meso-2,3-dimercaptosuccinic acid (DMSA) against the effects of lead acetate on oxidative stress and lead burden in rats.
The biochemical parameters and enzymes in different treated rats were determined by commercial kits. The metal concentrations were measured using atomic absorption spectrophotometer.
SGE (300 mg/kg) showed very low toxicity to organs in non-lead exposed rats. Administration of SGE individually had no effect on blood zinc protoporphyrin (ZPP) level but significantly enhanced the glutathione (GSH) content and delta-aminolevulinic acid dehydratase (ALAD), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in lead exposed rats. The co-treatment of SGE and DMSA had a synergism in increasing brain, liver and kidney superoxide dismutase (SOD), catalase (CAT) activities and GSH level, and decreasing oxidized glutathione (GSSG) and thiobarbituric acid reactive substances (TBARS) levels. Moreover, the co-treatment could improve the hepatic and renal histopathology changes. SGE as chelating agent showed significant efficiency in reducing blood and tissue lead burden.
The in vivo results suggested that SGE individually or combined with DMSA exhibited remarkable protective effects on lead-induced oxidative stress and lead burden in rats.
菝葜是一种传统的中国草药，其根茎在民间医学中被用于治疗铅中毒。
本研究旨在探讨菝葜提取物（SGE）单独或与meso-2,3-二巯基丁二酸（DMSA）联合使用对醋酸铅对大鼠氧化应激和铅负荷的影响的保护作用。
通过商业试剂盒测定不同处理大鼠的生化参数和酶。使用原子吸收分光光度计测量金属浓度。
SGE（300mg/kg）在未暴露于铅的大鼠中对器官表现出极低的毒性。SGE 单独给药对血液锌原卟啉（ZPP）水平没有影响，但显著增强了暴露于铅的大鼠的谷胱甘肽（GSH）含量和δ-氨基-酮戊酸脱水酶（ALAD）、丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP）活性。SGE 和 DMSA 的联合治疗在增加大脑、肝脏和肾脏超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和 GSH 水平，降低氧化型谷胱甘肽（GSSG）和硫代巴比妥酸反应物质（TBARS）水平方面具有协同作用。此外，联合治疗可以改善肝和肾的组织病理学变化。作为螯合剂的 SGE 显示出显著降低血液和组织中铅负荷的效率。
体内结果表明，SGE 单独或与 DMSA 联合使用对铅诱导的大鼠氧化应激和铅负荷具有显著的保护作用。
