Ben Alaya Walid, Sfar Imen, Aouadi Houda, Jendoubi Saloua, Najjar Tawfik, Filali Azza, Gorgi Yousr, Ben Abdallah Taieb, Mouelhi Leila, Matri Samira, Ayed Khaled
Charles Nicolle Hospital, Tunis-1006, Tunis, Tunisia.
Saudi J Gastroenterol. 2009 Jan;15(1):29-34. doi: 10.4103/1319-3767.43285.
BACKGROUND/AIM: To investigate the possible association between the polymorphism of the CTLA-4 exon 1 +49 A/G and susceptibility to Crohn's disease (CD) and ulcerative colitis (UC) in the Tunisian population.
The +49 A/G dimorphism was analyzed in 119 patients with CD, 65 patients with UC, and 100 controls by the polymerase chain reaction-restriction fragment length polymorphism method.
Significantly higher frequencies of the CTLA-4 +49A allele and A/A homozygous individuals were observed in patients with CD when compared with controls (pc = 0.0023 and pc = 0.0003, respectively). Analysis of CTLA-4 A/G polymorphism with respect to sex in CD showed a significant difference in A/A genotypes between female patients and controls (pc = 0.0001 and pc = 0.038, respectively). There were no differences in the subgroups of patients with CD.
Forty-nine A alleles and AA genotype are associated with CD susceptibility in Tunisians. Other genes involved in the T-cell regulation remain strong candidates for IBD susceptibility and require further investigation.
背景/目的：探讨突尼斯人群中细胞毒性T淋巴细胞相关抗原4（CTLA-4）外显子1 +49A/G多态性与克罗恩病（CD）及溃疡性结肠炎（UC）易感性之间的可能关联。
采用聚合酶链反应-限制性片段长度多态性方法，对119例CD患者、65例UC患者及100例对照者进行+49A/G二态性分析。
与对照组相比，CD患者中CTLA-4 +49A等位基因及A/A纯合个体的频率显著更高（分别为pc = 0.0023和pc = 0.0003）。对CD患者按性别分析CTLA-4 A/G多态性，结果显示女性患者与对照组之间A/A基因型存在显著差异（分别为pc = 0.0001和pc = 0.038）。CD患者各亚组之间无差异。
在突尼斯人中，49A等位基因和AA基因型与CD易感性相关。其他参与T细胞调节的基因仍是炎性肠病易感性的有力候选基因，需要进一步研究。
