Lankarani Kamran B, Karbasi Ashraf, Kalantari Tahereh, Yarmohammadi Hooman, Saberi-Firoozi Mehdi, Alizadeh-Naeeni Mahvash, Taghavi Ali R, Fattahi Mahammad R, Ghaderi Abbas
Gastroenterohepatology Research Center, Nemazee Hospital, Shiraz, Iran.
J Gastroenterol Hepatol. 2006 Feb;21(2):449-53. doi: 10.1111/j.1440-1746.2005.03956.x.
Ulcerative colitis (UC) is a multifactorial disease associated with dysregulated immunity. Recently, cytotoxic T lymphocyte associated antigen 4 (CTLA-4) gene polymorphisms have been reported in association with several autoimmune diseases in several populations. In the present study, the possible implication of the CTLA-4 gene as a risk factor for UC in the Iranian population was investigated.
One hundred UC patients and 100 healthy subjects were studied. CTLA-4 exon 1 position 49 (A/G: codon 17: Thr/Ala) polymorphisms were investigated by polymerase chain reaction single strand confirmation polymorphism method. Four of the patients and one of the healthy controls were excluded from the study because of incomplete DNA extraction.
The allele frequencies of A and G in 96 patients (A: 66.1%; G: 33.9%) were not significantly different from the 99 control subjects (A: 63.1%; G: 36.9%, P > 0.05). No significant differences in the distribution of genotype frequencies were observed between A + 49G gene polymorphisms and UC in the Iranian population (P > 0.05).
CTLA-4 polymorphism is not associated with UC in the Iranian population.
溃疡性结肠炎（UC）是一种与免疫调节失调相关的多因素疾病。最近，在多个群体中，细胞毒性T淋巴细胞相关抗原4（CTLA-4）基因多态性已被报道与多种自身免疫性疾病有关。在本研究中，我们调查了CTLA-4基因作为伊朗人群UC危险因素的潜在影响。
研究了100例UC患者和100例健康受试者。采用聚合酶链反应单链构象多态性方法检测CTLA-4外显子1第49位（A/G：密码子17：苏氨酸/丙氨酸）多态性。由于DNA提取不完全，4例患者和1例健康对照被排除在研究之外。
96例患者中A和G等位基因频率（A：66.1%；G：33.9%）与99例对照受试者（A：63.1%；G：36.9%，P>0.05）无显著差异。在伊朗人群中，A+49G基因多态性与UC之间的基因型频率分布无显著差异（P>0.05）。
在伊朗人群中，CTLA-4多态性与UC无关。
