Behrens W A, Depocas F
Can J Physiol Pharmacol. 1977 Jun;55(3):695-9. doi: 10.1139/y77-094.
Dopamine-beta-hydroxylase (DBH) (EC 1.14.17.1) activity is present in the interscapular brown adipose tissue (IBAT) as early as 2 days of age in the white rat. The specific and the total activities of this enzyme, as well as those of cytochrome oxidase (COX) (EC 1.9.3.1) in IBAT increase up to at least 20 days of age. Daily administration of 6-hydroxydopamine (6-OHDA) between the second and the twelfth day after birth does not significantly alter IBAT weight gain relative to untreated controls, but the increase in protein content with age is reduced to about half the normal value at the end of the treatment. The treatment with 6-OHDA also results in a drastic lowering of DBH specific and total activities, and a much smaller rate of increase of COX specific and total activities with age in IBAT compared with controls. These results provide additional evidence for a previously proposed role of sympathetic nervous system activity in the development of the thermogenic potential of IBAT in the newborn rat.
早在出生2天时，白色大鼠肩胛间棕色脂肪组织（IBAT）中就存在多巴胺-β-羟化酶（DBH）（EC 1.14.17.1）活性。该酶以及IBAT中细胞色素氧化酶（COX）（EC 1.9.3.1）的比活性和总活性在至少20日龄前均会增加。在出生后第2天至第12天期间每日给予6-羟基多巴胺（6-OHDA），相对于未处理的对照组，IBAT体重增加无显著变化，但在处理结束时，蛋白质含量随年龄的增加减少至正常值的约一半。与对照组相比，6-OHDA处理还导致IBAT中DBH的比活性和总活性大幅降低，以及COX的比活性和总活性随年龄增加的速率大幅降低。这些结果为先前提出的交感神经系统活动在新生大鼠IBAT产热潜能发育中的作用提供了额外证据。
