Skala J P, Hahn P
Can J Physiol Pharmacol. 1977 Apr;55(2):272-8. doi: 10.1139/y77-039.
A single-dose administration of 6-hydroxydopamine (50 mg/kg body weight, sc) to infant rats resulted in an enlargement, higher fresh weight, markedly elevated lipid content, and higher total protein content of their interscapular and cervical brown adipose tissue. The protein kinase (EC 2.7.1.37) activation ratio in the tissue was decreased as was the phosphoenolpyruvate carboxykinase (EC 4.1.1.32) activity. Fatty acid synthetase, on the other hand, showed an increased activity. These changes commenced as soon as 24 h after the administration of the drug, were fully developed 2-4 days later, and persisted for at least 14 days. The results are in line with the assumption that 6-hydroxydopamine administration causes chemical sympathectomy of brown adipose tissue. This is further supported by the fact that treatment with alpha-methyltyrosine, which is known to competively inhibit norepinephrine systhesis, results in similar changes in brown fat of infant rats. Hence it seems that 6-hydroxydopamine administration offers a simple and inexpensive experimental model for studies of the role norepinephrine-mediated sympathetic nervous system in brown tissue function and development.
给幼鼠单次皮下注射6-羟基多巴胺（50毫克/千克体重）会导致其肩胛间和颈部棕色脂肪组织增大、鲜重增加、脂质含量显著升高以及总蛋白含量升高。该组织中的蛋白激酶（EC 2.7.1.37）激活率降低，磷酸烯醇丙酮酸羧激酶（EC 4.1.1.32）活性也降低。另一方面，脂肪酸合成酶的活性增加。这些变化在给药后24小时内就开始出现，2 - 4天后完全显现，并持续至少14天。这些结果符合这样的假设，即给予6-羟基多巴胺会导致棕色脂肪组织的化学性交感神经切除术。已知竞争性抑制去甲肾上腺素合成的α-甲基酪氨酸处理幼鼠棕色脂肪会产生类似变化，这进一步支持了上述观点。因此，给予6-羟基多巴胺似乎为研究去甲肾上腺素介导的交感神经系统在棕色组织功能和发育中的作用提供了一个简单且廉价的实验模型。
