Depocas F, Foster D O, Zaror-Behrens G, Lacelle S, Nadeau B
Can J Physiol Pharmacol. 1984 Oct;62(10):1327-32. doi: 10.1139/y84-222.
Twenty-four hours after subcutaneous administration of 6-hydroxydopamine (6-OHDA) at a dose of 10 mg/kg body weight in warm-acclimated rats, noradrenaline (NA) content and dopamine beta-hydroxylase (DBH) activity of interscapular brown adipose tissue (IBAT) were reduced to about 10 and 35% of control values. Corresponding values for cold-acclimated rats, similarly treated, were 12 and 32%. In both groups of animals, calorigenic function in IBAT assessed by measurement of the effect of cold exposure on rate of blood flow through the tissue was lost almost completely. Twenty-four hours after treatment of rats with various doses of 6-OHDA, calorigenesis in IBAT was directly related to residual NA. Measurements of noradrenaline content and DBH activity from 1 to 10 days after 6-OHDA and the increase in IBAT blood flow of cold-exposed animals from 1 to 7 days after 6-OHDA indicated marked differences in the extent to which each of these indicators of the integrity and function of sympathetic nerve endings recovered with time and with the acclimation temperature of the animals. Regeneration of macromolecular components of noradrenergic vesicles in IBAT appeared to occur more rapidly in cold than in warm-acclimated animals. Thus, moderate doses of 6-OHDA may acutely sympathectomize IBAT, but as previously reported for other tissues, full recovery of function of IBAT occurs long before noradrenaline stores are replenished.
在暖适应大鼠皮下注射剂量为10mg/kg体重的6-羟基多巴胺（6-OHDA）24小时后，肩胛间棕色脂肪组织（IBAT）中的去甲肾上腺素（NA）含量和多巴胺β-羟化酶（DBH）活性降至对照值的约10%和35%。同样处理的冷适应大鼠的相应值分别为12%和32%。在两组动物中，通过测量冷暴露对组织血流速率的影响来评估的IBAT产热功能几乎完全丧失。用不同剂量的6-OHDA处理大鼠24小时后，IBAT中的产热与残余的NA直接相关。在注射6-OHDA后1至10天测量去甲肾上腺素含量和DBH活性，以及在注射6-OHDA后1至7天测量冷暴露动物的IBAT血流增加情况，结果表明，这些交感神经末梢完整性和功能指标随时间以及动物适应温度恢复的程度存在显著差异。IBAT中去甲肾上腺素能囊泡的大分子成分的再生在冷适应动物中似乎比在暖适应动物中发生得更快。因此，中等剂量的6-OHDA可能会急性去交感神经支配IBAT，但正如先前对其他组织的报道一样；在去甲肾上腺素储存得到补充之前很久，IBAT的功能就已完全恢复。
