Navarra Chiara O, Cadenaro Milena, Armstrong Steven R, Jessop Julie, Antoniolli Francesca, Sergo Valter, Di Lenarda Roberto, Breschi Lorenzo
Department of Biomedicine, University of Trieste, Trieste, Italy.
Dent Mater. 2009 Sep;25(9):1178-85. doi: 10.1016/j.dental.2009.05.009. Epub 2009 Jun 30.
To examine the degree of conversion (DC) of the adhesive interfaces created by Filtek Silorane Adhesive and Clearfil SE Bond using micro-Raman spectroscopy.
The adhesives were applied on human dentin in accordance with manufacturer's instructions. Specimens were cut to expose the bonded interfaces to the micro-Raman beam (Ranishaw InVia; laser wl 785 nm). Raman spectra were collected along the dentin/self-etching primer/adhesive interface at 1 microm intervals. The relative intensities of bands associated with mineral (P-O functional group at 960 cm(-1)) and adhesive (C-C-O group at 605 cm(-1)) components within the bonded interface were used to detect monomer penetration into the dentin matrix and to calculate the degree of conversion (C=C at 1640 cm(-1) as reaction peak, C-C-O at 605 cm(-1) as reference peak). Data were statistically analyzed with two-way ANOVA.
DC of Filtek Silorane Adhesive was 69+/-7% in the adhesive layer, increasing (p<0.05) to 93+/-5% in the primer and 92+/-9% in the hybrid layer. Clearfil SE Bond showed a DC of 83+/-3% in the hybrid and 85+/-3% in the adhesive layer. Thus, Filtek Silorane Adhesive showed a higher DC than Clearfil SE Bond in the hybrid layer (p<0.05), but a lower DC in the adhesive (p<0.05).
As high DC is a fundamental pre-requisite for the stability of the bond over time, this study supports the hypothesis that optimal stability of Filtek Silorane Adhesive can be obtained. However, further research is needed to investigate the mechanical properties of the hybrid layer created by Filtek Silorane Adhesive and its long-term stability.
使用显微拉曼光谱法检测Filtek Silorane粘接剂和Clearfil SE Bond所形成的粘接界面的转化程度（DC）。
按照制造商说明将粘接剂应用于人类牙本质。将样本切割，使粘接界面暴露于显微拉曼光束（Renishaw InVia；激光波长785 nm）下。沿着牙本质/自酸蚀底漆/粘接剂界面以1微米的间隔收集拉曼光谱。使用粘接界面内与矿物质（960 cm⁻¹处的P-O官能团）和粘接剂（605 cm⁻¹处的C-C-O基团）成分相关的谱带相对强度来检测单体渗入牙本质基质的情况，并计算转化程度（以1640 cm⁻¹处的C=C作为反应峰，605 cm⁻¹处的C-C-O作为参考峰）。数据采用双向方差分析进行统计学分析。
Filtek Silorane粘接剂在粘接剂层的DC为69±7%，在底漆层增加到93±5%（p<0.05），在混合层为92±9%。Clearfil SE Bond在混合层的DC为83±3%，在粘接剂层为85±3%。因此，Filtek Silorane粘接剂在混合层的DC高于Clearfil SE Bond（p<0.05），但在粘接剂层的DC较低（p<0.05）。
由于高DC是粘接随时间保持稳定性的基本前提条件，本研究支持这样的假设，即可以获得Filtek Silorane粘接剂的最佳稳定性。然而，需要进一步研究来探究Filtek Silorane粘接剂所形成的混合层的力学性能及其长期稳定性。
