Dubowitz Neuromuscular Centre, UCL Institute of Child Health, London, United Kingdom.
Stem Cells. 2009 Oct;27(10):2478-87. doi: 10.1002/stem.162.
Changes that occur in the skeletal muscle environment with the progress of muscular dystrophies may affect stem cell function and result in impaired muscle regeneration. It has previously been suggested that the success of stem cell transplantation could therefore be dependent both on the properties of the cell itself and on the host muscle environment. Here we engrafted young and mature adult mdx-nude mice, which are the genetic homolog of Duchenne muscular dystrophy, with a small number of satellite cells freshly isolated from young, normal donor mice. We found that the donor satellite cells contributed to muscle regeneration and self-renewal as efficiently within mature adult, as in young, dystrophic host muscle. Donor-derived satellite cells also contributed to robust regeneration after further injury, showing that they were functional despite the more advanced dystrophic muscle environment. These findings provide evidence that muscle tissue in a later stage of dystrophy may be effectively treated by stem cells.
随着肌肉疾病的进展，骨骼肌肉环境中发生的变化可能会影响干细胞的功能，导致肌肉再生受损。此前有人提出，干细胞移植的成功可能既取决于细胞本身的特性，也取决于宿主肌肉环境。在这里，我们将从小鼠体内分离出来的少量卫星细胞移植到年轻和成年的 mdx-nude 小鼠（Duchenne 肌营养不良症的遗传同源物）体内。我们发现，供体卫星细胞在成熟的成年宿主肌肉中与在年轻的、正常的供体肌肉中一样有效地促进肌肉再生和自我更新。供体来源的卫星细胞在进一步损伤后也有助于强有力的再生，这表明尽管肌肉环境更为严重，但它们仍具有功能。这些发现为肌肉疾病晚期的肌肉组织可能可以通过干细胞进行有效治疗提供了证据。
