Caligioni Claudia S
Cellular and Develomental Neurobiology, NIH/NINDS, Bethesda Maryland, USA.
Curr Protoc Neurosci. 2009 Jul;Appendix 4:Appendix 4I. doi: 10.1002/0471142301.nsa04is48.
The short reproductive cycle length observed in rodents, called the estrous cycle, makes them an ideal animal model for investigation of changes that occur during the reproductive cycle. Most of the data in the literature about the estrous cycle is obtained from rats because they are easily manipulated and they exhibit a clear and well-defined estrous cycle. However, the increased number of experiments using knockout mice requires identification of their estrous cycle as well, since (in)fertility issues may arise. In mice, like rats, the identification of the stage of estrous cycle is based on the proportion of cell types observed in the vaginal secretion. The aim of this unit is to provide guidelines for quickly and accurately determining estrous cycle phases in mice.
在啮齿动物中观察到的较短生殖周期长度，即发情周期，使其成为研究生殖周期中发生变化的理想动物模型。文献中关于发情周期的大多数数据是从大鼠获得的，因为它们易于操作，并且表现出清晰明确的发情周期。然而，使用基因敲除小鼠的实验数量不断增加，也需要确定它们的发情周期，因为可能会出现生育问题。在小鼠中，与大鼠一样，发情周期阶段的确定基于在阴道分泌物中观察到的细胞类型比例。本单元的目的是提供快速准确确定小鼠发情周期阶段的指南。
