Laboratory of Ontogeny and Reproduction, Centre Hospitalier Universitaire de Québec (CHUQ), Pavillon Centre Hospitalier de l'Université Laval (CHUL), Québec City, Québec, Canada.
Mol Cell Endocrinol. 2009 Nov 13;311(1-2):109-19. doi: 10.1016/j.mce.2009.06.012. Epub 2009 Jul 1.
A QPCR analysis of androgen receptor and several androgen metabolizing genes was performed during the saccular and alveolar stages of mouse lung development. Androgen receptor expression showed a statistically significant increase during the alveolar stage while levels of 17beta-hydroxysteroid dehydrogenase type 2 (17beta-HSD 2) expression significantly decreased at the end of the saccular stage and remained low throughout the alveolar period. 17beta-HSD 1, 17beta-HSD 5, 5alpha-reductase type 1, and mouse 3alpha-HSD did not present such a regulation. The androgen receptor protein was primarily detected in the nucleus of airway epithelial cells and of a subset of respiratory epithelial cells. 17beta-HSD 2 mRNA co-localized with androgen receptor protein during saccularization, but was absent from airway epithelium during alveolarization. Taken together, our results demonstrate temporal and spatial regulation of androgen receptor and 17beta-HSD 2 during the sacculo-alveolar transition period of mouse lung development suggesting control of androgen action.
在小鼠肺发育的囊泡和肺泡阶段进行了雄激素受体和几种雄激素代谢基因的 QPCR 分析。雄激素受体的表达在肺泡阶段呈统计学显著增加，而 17β-羟类固醇脱氢酶 2 型（17β-HSD 2）的水平在囊泡阶段结束时显著降低，并在整个肺泡期保持较低水平。17β-HSD 1、17β-HSD 5、5α-还原酶 1 和小鼠 3α-HSD 没有这种调节。雄激素受体蛋白主要存在于气道上皮细胞和一部分呼吸上皮细胞的核内。在囊泡形成过程中，17β-HSD 2mRNA 与雄激素受体蛋白共定位，但在肺泡化过程中不存在于气道上皮细胞中。总之，我们的结果表明，雄激素受体和 17β-HSD 2 在小鼠肺发育的囊泡-肺泡过渡期间存在时空调节，提示雄激素作用的控制。
