超快瞬态吸收光谱学：原理及其在光合系统中的应用
Ultrafast transient absorption spectroscopy: principles and application to photosynthetic systems.
作者信息
Berera Rudi, van Grondelle Rienk, Kennis John T M
机构信息
Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, Amsterdam, The Netherlands.
出版信息
Photosynth Res. 2009 Aug-Sep;101(2-3):105-18. doi: 10.1007/s11120-009-9454-y. Epub 2009 Jul 4.
The photophysical and photochemical reactions, after light absorption by a photosynthetic pigment-protein complex, are among the fastest events in biology, taking place on timescales ranging from tens of femtoseconds to a few nanoseconds. The advent of ultrafast laser systems that produce pulses with femtosecond duration opened up a new area of research and enabled investigation of these photophysical and photochemical reactions in real time. Here, we provide a basic description of the ultrafast transient absorption technique, the laser and wavelength-conversion equipment, the transient absorption setup, and the collection of transient absorption data. Recent applications of ultrafast transient absorption spectroscopy on systems with increasing degree of complexity, from biomimetic light-harvesting systems to natural light-harvesting antennas, are presented. In particular, we will discuss, in this educational review, how a molecular understanding of the light-harvesting and photoprotective functions of carotenoids in photosynthesis is accomplished through the application of ultrafast transient absorption spectroscopy.
光合色素 - 蛋白质复合物吸收光后发生的光物理和光化学反应，是生物学中最快的事件之一，其发生时间尺度从几十飞秒到几纳秒不等。能产生飞秒持续时间脉冲的超快激光系统的出现，开辟了一个新的研究领域，并能够实时研究这些光物理和光化学反应。在此，我们对超快瞬态吸收技术、激光和波长转换设备、瞬态吸收装置以及瞬态吸收数据的采集进行基本描述。还介绍了超快瞬态吸收光谱在从仿生光捕获系统到自然光捕获天线等复杂度不断增加的系统上的最新应用。特别是，在这篇教育综述中，我们将讨论如何通过应用超快瞬态吸收光谱来实现对光合作用中类胡萝卜素的光捕获和光保护功能的分子理解。
Zotero 插件