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利用瞬态吸收和飞秒荧光寿命光谱研究黄素细胞色素C中的超快动力学

Ultrafast Dynamics in Flavocytochrome C by Using Transient Absorption and Femtosecond Fluorescence Lifetime Spectroscopy.

作者信息

Khakurel Krishna P, Fuertes Gustavo, Sipos Aron, Paragi Gábor, Dostal Jakub, Kloz Miroslav, Žoldák Gabriel, Andreasson Jakob, Tóth András

机构信息

Extreme Light Infrastructure ERIC, Dolni Brezany CZ-25241, Czech Republic.

Institute of Biotechnology Czech Academy of Sciences, Vestec CZ-25250, Czech Republic.

出版信息

J Phys Chem B. 2025 Apr 17;129(15):3731-3739. doi: 10.1021/acs.jpcb.4c05496. Epub 2025 Apr 8.

DOI:10.1021/acs.jpcb.4c05496
PMID:40199724
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12010331/
Abstract

Flavocytochrome c sulfide dehydrogenase (FCC) is an important enzyme of sulfur metabolism in sulfur-oxidizing bacteria, and its catalytic properties have been extensively studied. However, the ultrafast dynamics of FCC is not well understood. We present ultrafast transient absorption and fluorescence spectroscopy measurements to unravel the early events upon excitation of the heme and flavin chromophores embedded in the flavocytochrome c (FccAB) from the bacterium . The fluorescence kinetics of FccAB suggests that the majority of the photoexcited species decay nonradiatively within the first few picoseconds. Transient absorption spectroscopy supports these findings by suggesting two major dynamic processes in FccAB, internal conversion occurring in about 400 fs and the vibrational cooling occurring in about 4 ps, mostly affecting the heme moiety.

摘要

黄素细胞色素c硫化物脱氢酶（FCC）是硫氧化细菌中硫代谢的一种重要酶，其催化特性已得到广泛研究。然而，FCC的超快动力学尚未得到很好的理解。我们进行了超快瞬态吸收和荧光光谱测量，以揭示来自该细菌的黄素细胞色素c（FccAB）中嵌入的血红素和黄素发色团激发后的早期事件。FccAB的荧光动力学表明，大多数光激发物种在最初的几皮秒内通过非辐射方式衰减。瞬态吸收光谱支持了这些发现，表明FccAB中有两个主要的动力学过程，内部转换发生在约400飞秒，振动冷却发生在约4皮秒，主要影响血红素部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f86a/12010331/7f27bbee387b/jp4c05496_0009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f86a/12010331/7f27bbee387b/jp4c05496_0009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f86a/12010331/7f27bbee387b/jp4c05496_0009.jpg

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