Omrani MirDavood, Ansari Mohammad Hassan Khadem, Agaverdizadae Davood
Department of Genetic, Motahary Hospital, Kashani AVE, Urmia, Iran.
Pak J Biol Sci. 2009 Feb 15;12(4):373-7. doi: 10.3923/pjbs.2009.373.377.
In order to establish a rapid and stable method for diagnosis of Mycobacterium tuberculosis infection and minimize the side effects of delayed diagnosis on patients and health system, a cross sectional study was carried out. Since, the infection rate with this bacteria increasing and one of the reasons for this increase is long process of laboratory identification, therefore establishing new diagnosis methods could decrease disease rate. To achieve this aim, collected sputum and blood specimens from 50 patients with clinical suspicion of pulmonary tuberculosis were studied with both traditional, acid-fast stain (AFB) and culture method compare to Enzyme-linked immunosorbent assay (Elisa) (IgG and IgM) and Polymerase Chain Reaction (PCR) methods. The sensitivity and specificity of all methods were determined by using the PCR results as the gold standard. The overall sensitivity, specificity, positive predictive value and negative predictive value of AFB were 17.64, 100, 100 and 70.12%. These values for culture method was 29.41, 100, 100 and 73.33% and for IgG antibody were 66.7, 81.81, 64.7 and 81.81% and IgM antibody were 70.58, 90.9, 80 and 85.71%, respectively. It was concluded that maximum sensitivity and specificity can be achieved by PCR method.
为建立一种快速、稳定的结核分枝杆菌感染诊断方法，并尽量减少延迟诊断对患者和卫生系统的副作用，开展了一项横断面研究。由于这种细菌的感染率在上升，而上升的原因之一是实验室鉴定过程漫长，因此建立新的诊断方法可以降低发病率。为实现这一目标，收集了50例临床怀疑患有肺结核患者的痰液和血液标本，采用传统的抗酸染色（AFB）和培养方法，并与酶联免疫吸附测定（ELISA）（IgG和IgM）以及聚合酶链反应（PCR）方法进行比较。以PCR结果作为金标准，确定所有方法的敏感性和特异性。AFB的总体敏感性、特异性、阳性预测值和阴性预测值分别为17.64%、100%、100%和70.12%。培养方法的这些值分别为29.41%、100%、100%和73.33%，IgG抗体分别为66.7%、81.81%、64.7%和81.81%，IgM抗体分别为70.58%、90.9%、80%和85.71%。结论是，PCR方法可实现最高的敏感性和特异性。
