利用非线性现象对线虫的细胞形态和细胞过程进行体内成像。
In vivo imaging of cell morphology and cellular processes in Caenorhabditis elegans, using non-linear phenomena.
作者信息
Filippidis G, Gualda E J, Mari M, Troulinaki K, Fotakis C, Tavernarakis N
机构信息
Institute of Electronic Structure and Laser, Foundation of Research and Technology-Hellas, P.O. Box 1385, 71110 Heraklion, Crete, Greece.
出版信息
Micron. 2009 Dec;40(8):876-80. doi: 10.1016/j.micron.2009.06.005. Epub 2009 Jun 21.
We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using non-linear microscopy. Complementary information about the anatomy of the nematode was collected by implementing a combination of two photon excitation fluorescence (TPEF), second and third harmonic generation (SHG and THG) image contrast modes on the same microscope. Three-dimensional (3D) reconstructions of TPEF, SHG and THG images were also performed. Moreover, THG imaging technique has been tested as a potential, novel, non-destructive diagnostic tool for monitoring cellular processes in vivo, such as neuronal degeneration.
我们使用非线性显微镜展示了秀丽隐杆线虫（C. elegans）的结构和过程的详细成像。通过在同一显微镜上结合使用双光子激发荧光（TPEF）、二次谐波和三次谐波产生（SHG和THG）图像对比模式，收集了有关线虫解剖结构的补充信息。还对TPEF、SHG和THG图像进行了三维（3D）重建。此外，THG成像技术已作为一种潜在的新型非破坏性诊断工具进行了测试，用于监测体内细胞过程，如神经元退化。
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