Bixel Gabriele M, Fretham Stephanie J B, Aschner Michael
Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, and University of Muenster, Muenster, Germany.
Department of Biology, Luther College, Decorah, Iowa.
Curr Protoc Toxicol. 2015 May 4;64(1119):11.19.1-11. doi: 10.1002/0471140856.tx1119s64.
In this protocol, we combine two-photon excitation fluorescence with nonlinear optical measurements to reconstruct the three-dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid-body region of Caenorhabditis elegans (C. elegans). Femto-second laser pulses excite second-harmonic generation (SHG) and third-harmonic generation (THG) signals, which show detailed structural information regarding the organization of myofibrils that are arranged around the central pharynx region. The combination of two-photon excitation with SHG and THG imaging is a very powerful tool to study cell morphology, microarchitecture, and tissue arrangement in C. elegans.
在本实验方案中，我们将双光子激发荧光与非线性光学测量相结合，以重建秀丽隐杆线虫（C. elegans）咽部区域以及身体前部和中部区域肌肉系统的三维结构。飞秒激光脉冲激发二次谐波产生（SHG）和三次谐波产生（THG）信号，这些信号显示了围绕中央咽部区域排列的肌原纤维组织的详细结构信息。双光子激发与SHG和THG成像相结合是研究秀丽隐杆线虫细胞形态、微观结构和组织排列的非常强大的工具。
