Maguis Séverine, Laffont Guillaume, Ferdinand Pierre, Carbonnier Benjamin, Kham Khémara, Mekhalif Tahar, Millot Marie-Claude
CEA, LIST, Laboratoire de Mesures Optiques, Centre d'Etudes de Saclay, Gif-sur-Yvette cedex, France.
Opt Express. 2008 Nov 10;16(23):19049-62. doi: 10.1364/oe.16.019049.
We present a study aimed at developing a label-free optical fiber biosensor for detection and quantification of biomolecules in real-time. The biosensor based on a Tilted Fiber Bragg Grating (TFBG) transduces a binding event between the probe and target molecules into a change in the refractive index of the medium surrounding the fiber. This work describes the experimental results obtained with three methods for immobilizing biomolecular probes on a TFBG silica cladding surface. Bovine serum albumin (BSA) and anti-BSA are used to assess the performances of the TFBG based biosensor in each configuration.
我们展示了一项旨在开发一种无标记光纤生物传感器的研究，用于实时检测和定量生物分子。基于倾斜光纤布拉格光栅（TFBG）的生物传感器将探针与目标分子之间的结合事件转化为光纤周围介质折射率的变化。这项工作描述了用三种将生物分子探针固定在TFBG二氧化硅包层表面的方法所获得的实验结果。牛血清白蛋白（BSA）和抗BSA用于评估每种配置下基于TFBG的生物传感器的性能。
