Proteomics and Microbiology Laboratory, University of Mons, Mons, Belgium; Electromagnetism and Telecommunication Department, University of Mons, Mons, Belgium.
Electromagnetism and Telecommunication Department, University of Mons, Mons, Belgium.
Biosens Bioelectron. 2016 Mar 15;77:315-22. doi: 10.1016/j.bios.2015.09.019. Epub 2015 Sep 12.
This study reports on the development of a surface plasmon resonance (SPR) optical fiber biosensor based on tilted fiber Bragg grating technology for direct detection of small biomarkers of interest for lung cancer diagnosis. Since SPR principle relies on the refractive index modifications to sensitively detect mass changes at the gold coated surface, we have proposed here a comparative study in relation to the target size. Two cytokeratin 7 (CK7) samples with a molecular weight ranging from 78 kDa to 2.6 kDa, respectively CK7 full protein and CK7 peptide, have been used for label-free monitoring. This work has first consisted in the elaboration and the characterization of a robust and reproducible bioreceptor, based on antibody/antigen cross-linking. Immobilized antibodies were then utilized as binding agents to investigate the sensitivity of the biosensor towards the two CK7 antigens. Results have highlighted a very good sensitivity of the biosensor response for both samples diluted in phosphate buffer with a higher limit of detection for the larger CK7 full protein. The most groundbreaking nature of this study relies on the detection of small biomolecule CK7 peptides in buffer and in the presence of complex media such as serum, achieving a limit of detection of 0.4 nM.
本研究报告了一种基于倾斜光纤布拉格光栅技术的表面等离子体共振（SPR）光纤生物传感器的开发，用于直接检测肺癌诊断中感兴趣的小生物标志物。由于 SPR 原理依赖于折射率的变化来敏感地检测金涂层表面的质量变化，因此我们在这里提出了与目标大小有关的比较研究。两种细胞角蛋白 7（CK7）样品，分子量分别为 78 kDa 至 2.6 kDa，分别为 CK7 全长蛋白和 CK7 肽，已被用于无标记监测。这项工作首先包括基于抗体/抗原交联的稳健且可重复的生物受体的制备和表征。然后将固定化抗体用作结合剂，以研究生物传感器对两种 CK7 抗原的灵敏度。结果表明，该生物传感器对两种在磷酸盐缓冲液中稀释的样品的响应具有很好的灵敏度，对于较大的 CK7 全长蛋白，检测限更高。这项研究最具开创性的性质在于在缓冲液中和在复杂介质（如血清）存在的情况下检测小分子生物标志物 CK7 肽，实现了 0.4 nM 的检测限。
