Wakamiya N, Osada Y, Wang D Q, Nakajima K, Kato S, Kurimura T, Naiki M
Department of Pathology, Osaka University, Japan.
Biochem Biophys Res Commun. 1991 Nov 27;181(1):310-5. doi: 10.1016/s0006-291x(05)81419-6.
Hanganutziu-Deicher (HD) antigen is a heterophile antigen that is widely distributed in many animals other than humans and chickens and is highly immunogenic in humans and chickens. In the present study, we demonstrated expression of HD-antigenic glycoproteins in activated T lymphocytes by SDS-PAGE and immunoblotting. Treatment with IL-2 plus PMA induced 29kD glycoprotein antigen detected under reducing condition. It contained sialic acid epitope of HD antigen because of the expression being neuraminidase-sensitive. Treatment with PMA plus A 23187 or PHA treatment and then PHA plus IL-2 treatment also induced two proteins of Mr 50kD and 70kD. These expressions were not detected in all individuals examined. These results indicate that HD antigen is an activated T cell antigen and expressed as an isoantigen as it is expressed in cancerous tissues from some patients.
汉加努齐乌-戴歇尔（HD）抗原是一种嗜异性抗原，广泛分布于除人类和鸡以外的许多动物中，在人类和鸡中具有高度免疫原性。在本研究中，我们通过SDS-PAGE和免疫印迹法证明了HD抗原性糖蛋白在活化T淋巴细胞中的表达。用IL-2加PMA处理可诱导在还原条件下检测到的29kD糖蛋白抗原。由于其表达对神经氨酸酶敏感，它含有HD抗原的唾液酸表位。用PMA加A 23187处理或PHA处理，然后PHA加IL-2处理也诱导出Mr为50kD和70kD的两种蛋白质。在所有检测的个体中均未检测到这些表达。这些结果表明，HD抗原是一种活化的T细胞抗原，并且作为一种同种抗原表达，就像在一些患者的癌组织中表达一样。
