Micó Vicente, Zalevsky Zeev, Ferreira Carlos, García Javier
Departamento de Optica, Universitat de Valencia, Burjassot, Spain.
Opt Express. 2008 Nov 10;16(23):19260-70. doi: 10.1364/oe.16.019260.
An approach that allows superresolution imaging of three-dimensional (3-D) samples by numerical refocusing is presented in the field of digital holographic microscopy. Based on the object's spectrum shift produced by tilted illumination, we present a time multiplexing superresolved approach to overcome the Abbe's diffraction limit. The proposed approach uses a microscope in a Mach-Zehnder interferometric architecture with the particularity that the output plane does not coincide with the image plane. Thus, a set of off-axis non-image plane holograms are sequentially recorded for every tilted beam used in the illumination stage. After that and by using simple digital post-processing and numerical reconstruction, a 3-D superresolved sample volume is reconstructed slice-by- slice in terms of the definition of a synthetic aperture (SA) that expands the cutoff frequency of the microscope lens. Experimental results showing the capabilities of the proposed approach are presented.
数字全息显微镜领域提出了一种通过数值重聚焦实现三维（3-D）样本超分辨率成像的方法。基于倾斜照明产生的物体光谱偏移，我们提出了一种时间复用超分辨率方法来克服阿贝衍射极限。所提出的方法使用马赫-曾德尔干涉结构的显微镜，其特殊之处在于输出平面与图像平面不重合。因此，针对照明阶段使用的每个倾斜光束，依次记录一组离轴非图像平面全息图。之后，通过简单的数字后处理和数值重建，根据扩展显微镜镜头截止频率的合成孔径（SA）定义，逐片重建三维超分辨率样本体积。给出了显示所提方法能力的实验结果。
