Brisco M J, Condon J, Sykes P J, Neoh S H, Morley A A
Department of Haematology, Flinders Medical Centre, Bedford Park, Australia.
Br J Haematol. 1991 Oct;79(2):211-7. doi: 10.1111/j.1365-2141.1991.tb04524.x.
We report a simple and robust method for sensitive quantitation of leukaemic cells in acute lymphocytic leukaemia. Chain determining region 3 (CDR3) of the immunoglobulin heavy chain gene is a precise genetic marker for a patient's leukaemic clone. Quantitation of the leukaemic lymphocytes was achieved by use of the polymerase chain reaction to detect CDR3 at limiting dilution of DNA samples. Five patients were studied and high levels (1 in 1 to 1 in 10) of leukaemic cells were detected at diagnosis or relapse. Leukaemic cells were detected in remission marrows from three patients, at levels of 1 in 1000 to 1 in 100,000. All five patients showed a 1000 to 100,000-fold reduction in the levels of leukaemic cells after induction therapy. This technique should prove useful for monitoring therapy and may help predict outcome.
我们报告了一种简单且可靠的方法，用于急性淋巴细胞白血病中白血病细胞的灵敏定量分析。免疫球蛋白重链基因的互补决定区3（CDR3）是患者白血病克隆的精确遗传标记。通过在DNA样本有限稀释的情况下使用聚合酶链反应检测CDR3，实现了白血病淋巴细胞的定量分析。对5名患者进行了研究，在诊断或复发时检测到高水平（1/1至1/10）的白血病细胞。在3名患者的缓解骨髓中检测到白血病细胞，水平为1/1000至1/100000。所有5名患者在诱导治疗后白血病细胞水平均降低了1000至100000倍。该技术应被证明对监测治疗有用，并可能有助于预测结果。
