Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Phytother Res. 2010 Jan;24 Suppl 1:S56-62. doi: 10.1002/ptr.2903.
The methanolic extract of fresh tea leaves of Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae) venoms. These snake venom enzymes are responsible for the early effects of envenomation, such as local tissue damage and inflammation. The CS extract inhibited phospholipase A(2), proteases, hyaluronidase and L-amino acid oxidase in both venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities of the venoms in vivo. It is suggested that the inhibitory potential of the CS extract against local tissue damage induced by snake venoms may be attributed to complexation and chelation between the venom proteins and the phenolic contents of the extract.
新鲜茶叶的甲醇提取物（Theaceae）（CS）被检测出其具有抑制眼镜蛇（Elapidae）和烙铁头（Viperidae）毒液中具有水解活性的酶的潜力。这些蛇毒液酶负责毒液的早期作用，如局部组织损伤和炎症。CS 提取物通过体外中和抑制磷脂酶 A(2)、蛋白酶、透明质酸酶和 L-氨基酸氧化酶在两种毒液中的活性，并抑制毒液在体内的出血和皮肤坏死活性。这表明 CS 提取物对蛇毒液引起的局部组织损伤的抑制作用可能归因于毒液蛋白与提取物中酚类成分之间的络合和螯合。
