Yamashita K, Atari E, Watanabe K, Shinoda H, Yokozawa M
Division of Surgical Pathology, Kitasato East Hospital, Kitasato University.
Hum Cell. 1991 Jun;4(2):145-9.
Strain KE-24 of colonic cancer cells was established from human colonic cancer diagnosed histopathologically as poorly differentiated adenocarcinoma. Doubling time of the cancer cell line was 32.4 hrs, and the karyotype was 46, xy, t (1q:?), 6p-, 14q+. The cancer cells could be heterotransplanted in 66% of nude mice. The plating efficiency was 17% in a soft agar plate with an inoculum size of 1 x 10(3) cells/dish. The tumor cells produced and released CEA and CA19-9 in the spent medium and these cancer cells were stained with both anti-CEA and anti-CA19-9 antibodies by the immunohistological staining method. Strain KE-24 of the cancer cells could be cultured in the serum-free medium (Media-I) for more than 100 passages.
结肠癌细胞系KE-24源自经组织病理学诊断为低分化腺癌的人类结肠癌。该癌细胞系的倍增时间为32.4小时，核型为46, xy, t (1q:?), 6p-, 14q+。66%的裸鼠能够异种移植该癌细胞。在接种量为1×10(3)个细胞/培养皿的软琼脂平板中，平板接种效率为17%。肿瘤细胞在培养液中产生并释放癌胚抗原（CEA）和糖类抗原19-9（CA19-9），并且通过免疫组织化学染色法，这些癌细胞用抗CEA和抗CA19-9抗体均可染色。癌细胞系KE-24能够在无血清培养基（培养基-I）中培养超过100代。
