Fullmer S C, Preshaw P M, Heasman P A, Kumar P S
Section of Periodontology, College of Dentistry, The Ohio State University, 4111 Postle Hall, 305 W. 12 Avenue, Columbus, OH 43210, USA.
J Dent Res. 2009 Jun;88(6):524-8. doi: 10.1177/0022034509338676.
Smoking cessation improves the clinical manifestations of periodontitis; however, its effect on the subgingival biofilm, the primary etiological agent of periodontitis, is unclear. The purpose of this study was to investigate, longitudinally, if smoking cessation altered the composition of the subgingival microbial community, by means of a quantitative, cultivation-independent assay for bacterial profiling. Subgingival plaque was collected at baseline, and 3, 6, and 12 months post-treatment from smokers who received root planing and smoking cessation counseling. The plaque was analyzed by terminal restriction fragment length polymorphism (t-RFLP). Microbial profiles differed significantly between smokers and quitters at 6 and 12 months following smoking cessation. The microbial community in smokers was similar to baseline, while quitters demonstrated significantly divergent profiles. Changes in bacterial levels contributed to this shift. These findings reveal a critical role for smoking cessation in altering the subgingival biofilm and suggest a mechanism for improved periodontal health associated with smoking cessation.
戒烟可改善牙周炎的临床表现；然而，其对牙周炎的主要病因——龈下生物膜的影响尚不清楚。本研究的目的是通过一种定量的、不依赖培养的细菌谱分析方法，纵向调查戒烟是否会改变龈下微生物群落的组成。在基线时以及接受根面平整和戒烟咨询的吸烟者治疗后3个月、6个月和12个月收集龈下菌斑。通过末端限制性片段长度多态性分析（t-RFLP）对菌斑进行分析。在戒烟后6个月和12个月，吸烟者和戒烟者的微生物谱存在显著差异。吸烟者的微生物群落与基线相似，而戒烟者则表现出明显不同的谱型。细菌水平的变化导致了这种转变。这些发现揭示了戒烟在改变龈下生物膜方面的关键作用，并提示了与戒烟相关的牙周健康改善机制。
