Takatalo M S, Tummers M, Thesleff I, Rönnholm R
University of Helsinki, Department of Biological and Environmental Sciences, Division of Biochemistry, P.O. Box 56 (Viikinkaari 5D), University of Helsinki FIN-00014, Finland.
J Dent Res. 2009 Jun;88(6):534-8. doi: 10.1177/0022034509338452.
GoPro49 is a recently identified, novel Golgi protein that is expressed in embryonic mesenchymal tissues, including dental follicle. In the present study, we have tested the hypothesis that the gene is a specific marker for the dental follicle, and examined its expression during the development of mouse incisors and molars. In situ hybridization showed that GoPro49 is expressed in dental follicles from bud to post-eruption stages. The expression is intense throughout the dental follicle during crown development, and persists in the root follicle during root development. In the forming periodontal ligament, GoPro49 expression is down-regulated upon differentiation of the follicle cells to cementoblasts and osteoblasts marked by Bsp1. In cultured dental follicle cells, the GoPro49 protein co-localizes with beta-COP, suggesting that GoPro49 may function in the secretory pathway. We conclude that GoPro49 is a novel, specific marker for the dental follicle and can be used to identify this tissue.
Bsp1, bone sialoprotein 1; GoPro49, Golgi protein 49 kDa; E16, embryonic day 16; HERS, Hertwig's epithelial root sheath; PDL, periodontal ligament; dpn, day post-natal.
GoPro49是一种最近鉴定出的新型高尔基体蛋白，在包括牙囊在内的胚胎间充质组织中表达。在本研究中，我们检验了该基因是牙囊特异性标志物的假说，并研究了其在小鼠门齿和臼齿发育过程中的表达情况。原位杂交显示，GoPro49在从牙蕾到萌出后阶段的牙囊中均有表达。在牙冠发育期间，整个牙囊的表达都很强烈，在牙根发育期间，根囊中的表达持续存在。在形成中的牙周韧带中，当牙囊细胞分化为以骨唾液酸蛋白1（Bsp1）为标记的成牙骨质细胞和成骨细胞时，GoPro49的表达下调。在培养的牙囊细胞中，GoPro49蛋白与β-COP共定位，提示GoPro49可能在分泌途径中发挥作用。我们得出结论，GoPro49是牙囊的一种新型特异性标志物，可用于识别该组织。
Bsp1，骨唾液酸蛋白1；GoPro49，49 kDa高尔基体蛋白；E16，胚胎第16天；HERS，赫特维希上皮根鞘；PDL，牙周韧带；dpn，出生后天数
