Endsley Janice J, Torres Alfredo G, Gonzales Christine M, Kosykh Valeri G, Motin Vladimir L, Peterson Johnny W, Estes D Mark, Klimpel Gary R
Department of Microbiology and Immunology , University of Texas Medical Branch, Galveston, TX 77555-0436, USA.
Open Microbiol J. 2009 Jun 5;3:92-6. doi: 10.2174/1874285800903010092.
Granulysin is a cationic protein produced by human T cells and natural killer cells that can kill bacterial pathogens through disruption of microbial membrane integrity. Herein we demonstrate antimicrobial activity of the granulysin peptide derived from the active site against Bacillus anthracis, Yersinia pestis, Francisella tularensis, and Burkholderia mallei, and show pathogen-specific differences in granulysin peptide effects. The susceptibility of Y. pestis to granulysin is temperature dependent, being less susceptible when grown at the flea arthropod vector temperature (26°C) than when grown at human body temperature. These studies suggest that augmentation of granulysin expression by cytotoxic lymphocytes, or therapeutic application of granulysin peptides, could constitute important strategies for protection against select agent bacterial pathogens. Investigations of the microbial surface molecules that determine susceptibility to granulysin may identify important mechanisms that contribute to pathogenesis.
颗粒溶素是一种由人类T细胞和自然杀伤细胞产生的阳离子蛋白，它可以通过破坏微生物膜的完整性来杀死细菌病原体。在此，我们证明了源自活性位点的颗粒溶素肽对炭疽芽孢杆菌、鼠疫耶尔森菌、土拉弗朗西斯菌和鼻疽伯克霍尔德菌具有抗菌活性，并显示了颗粒溶素肽作用的病原体特异性差异。鼠疫耶尔森菌对颗粒溶素的敏感性取决于温度，在跳蚤节肢动物载体温度（26°C）下生长时比在人体温度下生长时更不易感。这些研究表明，细胞毒性淋巴细胞增强颗粒溶素表达或颗粒溶素肽的治疗应用可能构成针对特定病原体细菌病原体的重要保护策略。对决定对颗粒溶素敏感性的微生物表面分子的研究可能会确定有助于发病机制的重要机制。
