Liu Yong Feng, Zan Lin Sen, Li Kui, Zhao Shuan Ping, Xin Ya Ping, Lin Qing, Tian Wan Qiang, Wang Zhi Wei
College of Animal Science and Technology, Northwest A & F University, Shaanxi, People's Republic of China.
Mol Biol Rep. 2010 Jan;37(1):429-34. doi: 10.1007/s11033-009-9604-5. Epub 2009 Jul 10.
Body measurement traits, influenced by genes and environmental factors, play numerous important roles in the value assessment of productivity and economy. Growth differentiate factor 5 (GDF5), involved in the development and maintenance of bone and cartilage, is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). In this study, based on the PCR-RFLP technology, we discovered and evaluated the potential association of the single nucleotide polymorphism (SNP) (T586C in exon 1) of the bovine GDF5 gene with body measurement traits in 985 Bos taurus breed, 42 Bos indicus breed and 76 Bos indicus x Bos taurus individuals. As the SNP marker, there were the significant effects on the Body length (BL) in the Bos taurus (BT) and Bos indicus x Bos taurus (BMY) populations (P < 0.05). In BT population, animals with the genotype TT had lower mean values for BL and Hip width (HW) than these with the TC and CC genotype (P < 0.01). In BMY population, animals with the genotype TC had lower mean values for BL than these with the genotype CC (P < 0.05). These results suggest that the SNP of the GDF5 gene could be a very useful genetic marker for body measurement traits in the bovine reproduction and breeding.
受基因和环境因素影响的体尺性状在生产力和经济价值评估中发挥着众多重要作用。生长分化因子5（GDF5）参与骨骼和软骨的发育与维持，是通过标记辅助选择（MAS）进行体尺性状选择的重要候选基因。在本研究中，基于PCR-RFLP技术，我们在985头荷斯坦牛品种、42头印度瘤牛品种和76头印度瘤牛×荷斯坦牛个体中发现并评估了牛GDF5基因单核苷酸多态性（SNP）（外显子1中的T586C）与体尺性状的潜在关联。作为SNP标记，其对荷斯坦牛（BT）群体和印度瘤牛×荷斯坦牛（BMY）群体的体长（BL）有显著影响（P < 0.05）。在BT群体中，基因型为TT的动物的BL和臀宽（HW）平均值低于基因型为TC和CC的动物（P < 0.01）。在BMY群体中，基因型为TC的动物的BL平均值低于基因型为CC的动物（P < 0.05）。这些结果表明，GDF5基因的SNP可能是牛繁殖和育种中体尺性状非常有用的遗传标记。
