The Key Laboratory of National Beef Cattle Improvement Center, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China.
Mol Biol Rep. 2011 Jan;38(1):411-6. doi: 10.1007/s11033-010-0123-1. Epub 2010 Mar 26.
The correlations between Y chromosome polymorphisms and the carcass traits were studied in five Chinese beef cattle populations by PCR, single strand conformation polymorphism and Y-STR sequence analysis. Nine alleles and their frequencies were identified on Y-STR UMN0929 region in Qinchuan (n=116), Luxi (n=112), Jinnan (n=104) pure breeds, Simmental×Qinchuan crossbred (n=80) and Angus×Qinchuan crossbred (n=96). The most popular A-176 and B-178 alleles were presented in all 5 cattle populations in the range of 12% (Jinnan) to 66% (Simmental×Qinchuan). The allele I-194 presented Luxi and Angus×Qinchuan. In Qinchun cattle, G-190 and E-186 alleles had bigger effect on BPI (4.23±0.32 and 4.22±0.48 kg/cm, P<0.01) and CW (325.40±49.42 and 316.73±45.29 kg, P<0.01), respectively. In Luxi cattle, I-194 allele affected higher BPI (4.08±0.35 kg/cm, P<0.01) and CW (302.07±17.55 kg, P<0.01), respectively. In Jinnan cattle breed, H-192 had higher BPI (4.32±0.50 kg/cm, P<0.05) and CW (327.87±59.37 kg, P<0.05), respectively. In Simmental×Qinchuan cross breed, C-180 allele affected largely on BPI (5.16±0.25 kg/cm, P<0.05) and CW (393.16±25.92 kg, P<0.05). In Angus×Qinchuan cross breed, I-194 had higher BPI (4.43±0.33 kg, P<0.05) and CW (346.63±29.77 kg, P<0.05). Correlations between alleles and other carcass traits (net meat weight, top grade weight, slaughter rate, net meat rate, loin-eye muscle area, carcass length, meet tenderness and shear force) were also analyzed using mixed-effect model. Cattle Y-STR UMN0929 loci alleles and its correlation with carcass traits in beef cattle populations could be implemented into the cattle breeding program for choosing beef cattle with better carcass traits.
通过 PCR、单链构象多态性和 Y-STR 序列分析，研究了 5 个中国肉牛群体中 Y 染色体多态性与胴体性状的相关性。在秦川（n=116）、鲁西（n=112）、晋南（n=104）纯种种群、西门塔尔×秦川杂交牛（n=80）和安格斯×秦川杂交牛（n=96）的 Y-STR UMN0929 区域，鉴定了 9 个等位基因及其频率。最受欢迎的 A-176 和 B-178 等位基因在所有 5 个牛群中的频率范围为 12%（晋南）至 66%（西门塔尔×秦川）。在鲁西牛和安格斯×秦川牛中存在 I-194 等位基因。在秦川牛中，G-190 和 E-186 等位基因对 BPI（4.23±0.32 和 4.22±0.48 kg/cm，P<0.01）和 CW（325.40±49.42 和 316.73±45.29 kg，P<0.01）有较大影响。在鲁西牛中，I-194 等位基因对 BPI（4.08±0.35 kg/cm，P<0.01）和 CW（302.07±17.55 kg，P<0.01）有较大影响。在晋南牛品种中，H-192 对 BPI（4.32±0.50 kg/cm，P<0.05）和 CW（327.87±59.37 kg，P<0.05）有较大影响。在西门塔尔×秦川杂交牛中，C-180 等位基因对 BPI（5.16±0.25 kg/cm，P<0.05）和 CW（393.16±25.92 kg，P<0.05）有较大影响。在安格斯×秦川杂交牛中，I-194 对 BPI（4.43±0.33 kg，P<0.05）和 CW（346.63±29.77 kg，P<0.05）有较大影响。利用混合效应模型还分析了等位基因与其他胴体性状（净肉重、特级肉重、屠宰率、净肉率、腰眼肌面积、胴体长、肉质嫩度和剪切力）之间的关系。牛 Y-STR UMN0929 基因座的等位基因及其与肉牛群体胴体性状的相关性可应用于牛的选育计划，以选择具有更好胴体性状的肉牛。
