Di Corato Riccardo, Piacenza Philomena, Musarò Mariarosaria, Buonsanti Raffaella, Cozzoli Pantaleo Davide, Zambianchi Massimo, Barbarella Giovanna, Cingolani Roberto, Manna Liberato, Pellegrino Teresa
National Nanotechnology Laboratory of CNR-INFM, Lecce, Italy.
Macromol Biosci. 2009 Oct 8;9(10):952-8. doi: 10.1002/mabi.200900154.
Nanostructures displaying fluorescence and magnetic properties at the same time are potentially useful for achieving simultaneous bio-separation and bio-sensing (e.g., magnetic separation coupled with multiplexing optical detection of different tumour cell populations). Spherical nanobeads that display both fluorescent and magnetic features are reported; they are fabricated by grafting fluorescent oligothiophene molecules to an amphiphilic polymer that is then used to enwrap iron oxide nanoparticles, which acts as the magnetic domain. By tuning experimental conditions, control over the number of magnetic nanoparticles per bead and over the bead diameter (30-400 nm) was achieved. A cell separation efficiency of the level required for cell sorting applications is also reported.
同时具有荧光和磁性的纳米结构对于实现同步生物分离和生物传感具有潜在的应用价值（例如，磁分离与不同肿瘤细胞群体的多重光学检测相结合）。有报道称制备出了兼具荧光和磁性特征的球形纳米珠；它们是通过将荧光低聚噻吩分子接枝到两亲聚合物上制备而成，然后用该聚合物包裹作为磁畴的氧化铁纳米颗粒。通过调整实验条件，可以控制每个纳米珠上磁性纳米颗粒的数量以及纳米珠的直径（30 - 400纳米）。文中还报道了细胞分选应用所需水平的细胞分离效率。
