CNR NANOTEC, Institute of Nanotechnology c/o Campus Ecotekne, Via Monteroni, 73100 Lecce, Italy.
Italian Institute of Technology (IIT) , via Morego 30, 16163 Genova, Italy.
ACS Appl Mater Interfaces. 2017 Oct 11;9(40):35095-35104. doi: 10.1021/acsami.7b05709. Epub 2017 Sep 26.
In this work, the versatility of layer-by-layer technology was combined with the magnetic response of iron oxide nanobeads to prepare magnetic mesostructures with a degradable multilayer shell into which a dye quenched ovalbumin conjugate (DQ-OVA) was loaded. The system was specifically designed to prove the protease sensitivity of the hybrid mesoscale system and the easy detection of the ovalbumin released. The uptake of the nanostructures in the breast cancer cells was followed by the effective release of DQ-OVA upon activation via the intracellular proteases degradation of the polymer shells. Monitoring the fluorescence rising due to DQ-OVA digestion and the cellular dye distribution, together with the electron microscopy studying, enabled us to track the shell degradation and the endosomal uptake pathway that resulted in the release of the digested fragments of DQ ovalbumin in the cytosol.
在这项工作中，层层技术的多功能性与氧化铁纳米珠的磁响应相结合，制备了具有可降解多层壳的磁性介孔结构，将染料猝灭的卵清蛋白缀合物（DQ-OVA）载入其中。该系统专门用于证明杂交介观系统的蛋白酶敏感性以及对释放的卵清蛋白的易于检测。通过细胞内蛋白酶降解聚合物壳，纳米结构在乳腺癌细胞中的摄取，随后有效释放 DQ-OVA。由于 DQ-OVA 消化引起的荧光上升和细胞染料分布的监测，以及电子显微镜研究，使我们能够跟踪壳的降解和内体摄取途径，从而导致细胞质中 DQ 卵清蛋白消化片段的释放。
