Quarta Alessandra, Di Corato Riccardo, Manna Liberato, Argentiere Simona, Cingolani Roberto, Barbarella Giovanna, Pellegrino Teresa
National Nanotechnology Laboratory of CNR-INFM and IIT Research Unit, Via per Arnesano 16, Km 5, 73100, Lecce, Italy.
J Am Chem Soc. 2008 Aug 13;130(32):10545-55. doi: 10.1021/ja800102v. Epub 2008 Jul 16.
The combination of materials that possess different properties (such as, for instance, fluorescence and magnetism) into one single object of nanoscale size represents an attractive challenge for biotechnology, especially for their potential relevance in biomedical applications. We report here the preparation of novel bifunctional conjugates based on the linkage of inorganic nanoparticles to organic oligothiophene fluorophores (OTFs). In comparison to the organic dyes commonly used in bioimaging and more similarly to colloidal quantum dots, OTFs have broad optical absorption spectra, and therefore OTF fluorophores emitting at different colors can be excited with a single excitation source, allowing for easier multiplexing analysis. In this work we show the preparation of OTF-nanoparticle conjugates based on gold and iron oxide nanoparticles and their characterization using different techniques such as gel electrophoresis, photoluminescence spectroscopy, dynamic light scattering, and so on. In addition, by performing an in vitro study on human tumor cells we show that OTF-nanoparticle conjugates emitting at different colors can be used for multiplexing detection. Also, in the case of iron oxide-OTF conjugates, once uptaken by the cells, we show that they preserve both their fluorescent and their magnetic properties.
将具有不同性质（例如荧光和磁性）的材料组合成单个纳米级尺寸的物体，这对生物技术来说是一项具有吸引力的挑战，特别是考虑到它们在生物医学应用中的潜在相关性。我们在此报告基于无机纳米颗粒与有机低聚噻吩荧光团（OTF）连接制备新型双功能共轭物。与生物成像中常用的有机染料相比，更类似于胶体量子点，OTF具有宽光学吸收光谱，因此可以用单一激发源激发发射不同颜色的OTF荧光团，便于进行更轻松的多重分析。在这项工作中，我们展示了基于金和氧化铁纳米颗粒的OTF-纳米颗粒共轭物的制备及其使用不同技术（如凝胶电泳、光致发光光谱、动态光散射等）的表征。此外，通过对人肿瘤细胞进行体外研究，我们表明发射不同颜色的OTF-纳米颗粒共轭物可用于多重检测。而且，对于氧化铁-OTF共轭物，一旦被细胞摄取，我们表明它们保留了荧光和磁性特性。
