Suzuki Ryo, Hattori Fumiyuki, Itabashi Yuji, Yoshioka Masatoyo, Yuasa Shinsuke, Manabe-Kawaguchi Haruko, Murata Mitsushige, Makino Shinji, Kokaji Kiyokazu, Yozu Ryohei, Fukuda Keiichi
Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan.
Biochem Biophys Res Commun. 2009 Sep 18;387(2):353-9. doi: 10.1016/j.bbrc.2009.07.024. Epub 2009 Jul 10.
Myocardial cell sheets (MCS) are a potentially valuable tool for tissue engineering aimed at heart regeneration. Several methods have recently been established for the fabrication of MCS. However, the lack of a sufficient blood supply has inhibited functional recovery of the MCS. To address this challenge, we combined MCS transplantation with omentopexy (OP), which utilizes omental tissue as a surgical flap. Rats were divided into five groups: sham, myocardial infarction (MI), MCS transplantation, OP, and MCS+OP. Histologic analysis revealed that MCS+OP drastically reversed MI-induced cardiac remodeling. Echocardiography revealed that MCS increased cardiac function, while OP had a synergistic beneficial effect with MCS transplantation. Immunofluorescence imaging showed that OP increased the survival of transplanted cardiomyocytes, and increased the blood supply through enhancement of angiogenesis and migration of small arteries into the MCS. Taken together, we concluded that OP is a promising strategy for the enhancement of graft function in MCS transplantation.
心肌细胞片（MCS）是用于心脏再生组织工程的一种潜在有价值的工具。最近已经建立了几种制备MCS的方法。然而，缺乏充足的血液供应抑制了MCS的功能恢复。为应对这一挑战，我们将MCS移植与网膜固定术（OP）相结合，后者利用网膜组织作为手术皮瓣。将大鼠分为五组：假手术组、心肌梗死（MI）组、MCS移植组、OP组和MCS + OP组。组织学分析显示，MCS + OP显著逆转了MI诱导的心脏重塑。超声心动图显示，MCS提高了心脏功能，而OP与MCS移植具有协同有益作用。免疫荧光成像显示，OP提高了移植心肌细胞的存活率，并通过增强血管生成和小动脉向MCS的迁移增加了血液供应。综上所述，我们得出结论，OP是增强MCS移植中移植物功能的一种有前景的策略。
