Robins R J, Bachmann P, Robinson T, Rhodes M J, Yamada Y
Genetics and Microbiology Department, AFRC Institute of Food Research, Norwich Laboratory, UK.
FEBS Lett. 1991 Nov 4;292(1-2):293-7. doi: 10.1016/0014-5793(91)80887-9.
Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-ol) with acetyl-coenzyme A is also present. These two activities can be partially resolved by anion-exchange chromatography, some fractions containing only the pseudotropine-utilizing activity. The basic properties of these two enzymes are reported and their roles in forming the observed alkaloid spectrum of D. stramonium roots discussed.
托品（托烷-3α-醇）是阿托品形成过程中的中间体。在曼陀罗转化根培养物中发现了一种酰基转移酶活性，该活性可以使用乙酰辅酶A作为共底物将托品乙酰化。还存在另一种酰基转移酶活性，它能将伪托品（托烷-3β-醇）与乙酰辅酶A进行乙酰化反应。这两种活性可以通过阴离子交换色谱法部分分离，一些馏分仅含有利用伪托品的活性。本文报道了这两种酶的基本特性，并讨论了它们在形成曼陀罗根中观察到的生物碱谱中的作用。
