茉莉酸甲酯对曼陀罗毛状根液体培养中主要托烷生物碱积累与释放的影响

[Effects of methyl jasmonate on accumulation and release of main tropane alkaloids in liquid cultures of Datura stramonium hairy root].

作者信息

Sun Ji-Wei, Zhang Hong, Wang Feng-Ying, Sun Yi-Ming, Sun Min

机构信息

School of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, MOE, Chongqing 400715, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Jun;38(11):1712-8.

PMID:24010283

Abstract

OBJECTIVE

To study the effects of methyl jasmonate (MJ) on the accumulation and release of main secondary metabolites i. e. scopolamine and hyoscyamine in liquid cultures of Datura stramonium hairy roots.

METHOD

After 18 days liquid culture of D. stramonium hairy roots induced by agrobacterium rhizogenes C58C1, the chemical elicitor methyl jasmonate was added into 1/2 MS liquid cultures and scopolamine and hyoscyamine on the day 0, 3, 6, 9 and 12, after dealing with MJ, was determined by HPLC.

RESULT

After dealing with MJ on the day 3, 6, 9 and 12,the concentration of scopolamine reached to 0.419, 0.439, 0.431, 0.374 mg x g(-1), respectively, the increase of scopolamine were 1.36, 1.42, 1.17 and 1.12 fold higher than that of the control, respectively. And hyoscyamine reached 1.493, 0.817, 0.723 and 0.698 mg x g(-1), respectively, the increase of hyoscyamine were 2.28, 1.11, 0.63 and 0.70 fold higher than that of the control, respectively.

CONCLUSION

MJ could stimulate the accumulation of scopolamine and hyoscyamine (3,6 d) in D. stramonium hairy root and have released them into the culture medium.

摘要

目的

研究茉莉酸甲酯（MJ）对曼陀罗毛状根液体培养物中主要次生代谢产物即东莨菪碱和莨菪碱积累与释放的影响。

方法

用发根农杆菌C58C1诱导曼陀罗毛状根进行18天液体培养后，在第0、3、6、9和12天向1/2 MS液体培养基中添加化学诱导剂茉莉酸甲酯，处理MJ后，通过高效液相色谱法测定东莨菪碱和莨菪碱。

结果

在第3、6、9和12天用MJ处理后，东莨菪碱浓度分别达到0.419、0.439、0.431、0.374mg·g⁻¹，东莨菪碱的增加量分别比对照高1.36、1.42、1.17和1.12倍。莨菪碱分别达到1.493、0.817、0.723和0.698mg·g⁻¹，莨菪碱的增加量分别比对照高2.28、1.11、0.63和0.70倍。