Yin Mei-Chin, Lin Chun-Che, Wu Hsi-Chin, Tsao Shih-Ming, Hsu Cheng-Kuang
Department of Nutrition, China Medical University, Taichung City, Taiwan, ROC.
J Agric Food Chem. 2009 Jul 22;57(14):6468-73. doi: 10.1021/jf9004466.
Apoptotic effects of protocatechuic acid (PCA) at 1, 2, 4, 8 micromol/L on human breast cancer MCF7 cell, lung cancer A549 cell, HepG2 cell, cervix HeLa cell, and prostate cancer LNCaP cell were examined. Results showed that PCA concentration-dependently decreased cell viability, increased lactate dehydrogenase leakage, enhanced DNA fragmentation, reduced mitochondrial membrane potential, and lowered Na(+)-K(+)-ATPase activity for these cancer cells (P < 0.05). PCA also concentration-dependently elevated caspase-3 activity in five cancer cells (P < 0.05), but this agent at 2-8 micromol/L significantly increased caspase-8 activity (P < 0.05). PCA concentration-dependently decreased intercellular adhesion molecule level in test cancer cells (P < 0.05) but significantly inhibited cell adhesion at 2-8 micromol/L (P < 0.05). PCA also concentration-dependently lowered the levels of interleukin (IL)-6 and IL-8 in five cancer cells (P < 0.05), but this agent at 2-8 micromol/L significantly suppressed vascular endothelial growth factor production (P < 0.05). These findings suggest that PCA is a potent anticancer agent to cause apoptosis or retard invasion and metastasis in these five cancer cells.
检测了原儿茶酸（PCA）在1、2、4、8微摩尔/升浓度下对人乳腺癌MCF7细胞、肺癌A549细胞、肝癌HepG2细胞、宫颈癌HeLa细胞和前列腺癌LNCaP细胞的凋亡作用。结果显示，PCA对这些癌细胞具有浓度依赖性地降低细胞活力、增加乳酸脱氢酶泄漏、增强DNA片段化、降低线粒体膜电位以及降低钠钾ATP酶活性的作用（P<0.05）。PCA还能浓度依赖性地提高五种癌细胞中的半胱天冬酶-3活性（P<0.05），但该药物在2 - 8微摩尔/升时能显著增加半胱天冬酶-8活性（P<0.05）。PCA能浓度依赖性地降低受试癌细胞中的细胞间黏附分子水平（P<0.05），但在2 - 8微摩尔/升时能显著抑制细胞黏附（P<0.05）。PCA还能浓度依赖性地降低五种癌细胞中白细胞介素（IL）-6和IL-8的水平（P<0.05），但该药物在2 - 8微摩尔/升时能显著抑制血管内皮生长因子的产生（P<0.05）。这些发现表明，PCA是一种有效的抗癌药物，可导致这五种癌细胞凋亡或延缓其侵袭和转移。
