Yu Ching-Han, Kan Shu-Fen, Shu Chin-Hang, Lu Ting-Jang, Sun-Hwang Lucy, Wang Paulus S
Department of Physiology, School of Medicine, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan, Republic of China.
J Nutr Biochem. 2009 Oct;20(10):753-64. doi: 10.1016/j.jnutbio.2008.07.004. Epub 2008 Oct 16.
Agaricus blazei Murill (A. blazei) has been conventionally used as a health food for the prevention of cancer. However, little is known about the direct effects and action mechanisms of A. blazei on human prostate cancer. In the present study, the effects of A. blazei on the growth of human prostate cancer were examined in vitro and in vivo. A. blazei, especially the broth fraction, inhibited cell proliferation in both androgen-dependent and androgen-independent prostate cancer cell lines. The broth of A. blazei induced lactate dehydrogenase leakage in three cancer cell lines, whereas the activities of caspase 3 and the DNA fragmentation were enhanced the most in androgen-independent PC3 cells. The protein expressions of apoptosis-related molecules were elevated by the broth of A. blazei in PC3 cells. Oral supplementation with the broth of A. blazei (with the higher ratio of beta-glucan) significantly suppressed tumor growth without inducing adverse effects in severe combined immunodeficient mice with PC3 tumor xenograft. Tumor xenografts from A. blazei-fed mice showed decreased proliferating cell nuclear antigen-positive cells and reduced tumor microvessel density. Based on these results, we found that the broth of A. blazei may directly inhibit the growth of prostate cancer cell via an apoptotic pathway and suppress prostate tumor growth via antiproliferative and antiangiogenic mechanisms. We therefore suggest that A. blazei might have potential therapeutic use in the prevention and treatment of human prostate cancer.
姬松茸一直被传统地用作预防癌症的保健食品。然而，关于姬松茸对人类前列腺癌的直接作用及其作用机制却知之甚少。在本研究中，我们在体外和体内检测了姬松茸对人类前列腺癌生长的影响。姬松茸，尤其是其培养液成分，在雄激素依赖型和雄激素非依赖型前列腺癌细胞系中均能抑制细胞增殖。姬松茸培养液在三种癌细胞系中均诱导了乳酸脱氢酶泄漏，而在雄激素非依赖型PC3细胞中，半胱天冬酶3的活性和DNA片段化程度增强最为明显。姬松茸培养液使PC3细胞中凋亡相关分子的蛋白表达升高。在携带PC3肿瘤异种移植的严重联合免疫缺陷小鼠中，口服补充姬松茸培养液（β-葡聚糖比例较高）可显著抑制肿瘤生长，且未引发不良反应。来自喂食姬松茸小鼠的肿瘤异种移植显示增殖细胞核抗原阳性细胞减少，肿瘤微血管密度降低。基于这些结果，我们发现姬松茸培养液可能通过凋亡途径直接抑制前列腺癌细胞的生长，并通过抗增殖和抗血管生成机制抑制前列腺肿瘤生长。因此，我们认为姬松茸在人类前列腺癌的预防和治疗中可能具有潜在的治疗用途。
