Hashimoto H, Ishikawa T, Yamaguchi K, Shiotani M, Fujisawa M
Division of Urology, Kobe University Graduate School of Medicine, Kobe, 650-z0017 Japan.
Andrologia. 2009 Aug;41(4):216-21. doi: 10.1111/j.1439-0272.2009.00918.x.
Testicular torsion causes ischaemia-reperfusion (I-R) injury of testis and might lead to male infertility. Its injury initiates a pathophysiological cascade, including an activation of inflammatory cytokines and generation of nitric oxide and other reactive oxygen species. Vascular endothelial growth factor (VEGF) mediates angiogenesis and promotes endothelial cell survival. The aim of our study was to investigate the time course expression of VEGF, VEGF-receptor (R)1, VEGF-R2, nitric oxide synthases (NOS) in experimental I-R injury of rat testis. In torsion side testis, the expression of VEGF protein and mRNA significantly increased in a time-dependent manner (P < 0.001 and P < 0.001, respectively). Although the expression of VEGF-R1 mRNA was increased in a similar way (P < 0.001), VEGF-R2 mRNA expression was not detected. In immunohistochemistry, the increase in VEGF protein staining was observed in testicular vascular endothelial cells and germ cells at 24 h after reperfusion. Significant activation of inducible NOS and endothelial NOS was investigated at 12 and 24 h after reperfusion (P < 0.01 and P < 0.001, respectively). This is the first report to show the time course expression of VEGF in experimental I-R rat testis.
睾丸扭转会导致睾丸缺血再灌注（I-R）损伤，并可能导致男性不育。其损伤引发了一系列病理生理级联反应，包括炎性细胞因子的激活以及一氧化氮和其他活性氧的产生。血管内皮生长因子（VEGF）介导血管生成并促进内皮细胞存活。我们研究的目的是调查VEGF、VEGF受体（R）1、VEGF-R2、一氧化氮合酶（NOS）在大鼠睾丸实验性I-R损伤中的时间进程表达。在扭转侧睾丸中，VEGF蛋白和mRNA的表达呈时间依赖性显著增加（分别为P < 0.001和P < 0.001）。虽然VEGF-R1 mRNA的表达以类似方式增加（P < 0.001），但未检测到VEGF-R2 mRNA的表达。在免疫组织化学中，再灌注24小时后在睾丸血管内皮细胞和生殖细胞中观察到VEGF蛋白染色增加。再灌注12小时和24小时后分别检测到诱导型NOS和内皮型NOS的显著激活（分别为P < 0.01和P < 0.001）。这是首次报道在实验性I-R大鼠睾丸中VEGF的时间进程表达。
