de Almeida Denize E, Roveratti Cristina, Brito Fábio L C, Godoy Guilherme S, Duque Juan C M, Bechara Gervásio H, Laus José L
Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Vet Ophthalmol. 2009 Jul-Aug;12(4):211-5. doi: 10.1111/j.1463-5224.2009.00699.x.
This study compared the histopathology of canine distemper virus (CDV)-induced keratoconjunctivitis sicca (KCS) to non-infectious KCS in conjunctival tissues.
Forty mongrel dogs were assigned to three distinct groups: (i) non-infectious KCS (G1, n = 10), (ii) CDV-induced KCS (G2, n = 20), and (iii) healthy animals without any ocular alterations (G3, n = 10).
IgG titers and physical and ophthalmic examinations (e.g. Schirmer tear test [STT], tonometry, biomicroscopy, indirect biomicroscopy, and fluorescein test) were performed on all dogs. Conjunctival biopsies were collected and examined microscopically.
Non-infectious and CDV-induced KCS demonstrated similar histopathological changes. Both types of KCS correlated with low STT, conjunctival hyperemia, mucopurulent ocular discharge, predominant lymphoplasmacytic infiltration, and acantholysis and keratinization of the ocular surface. G1 had lower conjunctival goblet cell counts than G3. Inclusion bodies were sporadically found in conjunctival samples of dogs from G2. The severity of ocular lesions in G1 and G2 did not correlate with the histopathological findings.
Dogs with non-infectious and CDV-induced KCS had very similar conjunctival histopathology. Our findings suggest that the pathophysiology of CDV-induced KCS is likely to be the same as non-infectious KCS, that is, a result of lacrimal deficiency and inflammation of the ocular surface.
本研究比较了犬瘟热病毒（CDV）引起的干眼病（KCS）与非感染性KCS结膜组织的组织病理学。
40只杂种犬被分为三个不同的组：（i）非感染性KCS（G1，n = 10），（ii）CDV诱导的KCS（G2，n = 20），以及（iii）无任何眼部改变的健康动物（G3，n = 10）。
对所有犬进行IgG滴度以及体格和眼科检查（例如泪液分泌试验[STT]、眼压测量、生物显微镜检查、间接生物显微镜检查和荧光素试验）。采集结膜活检组织并进行显微镜检查。
非感染性和CDV诱导的KCS表现出相似的组织病理学变化。两种类型的KCS均与低STT、结膜充血、黏液脓性眼分泌物、主要的淋巴浆细胞浸润以及眼表面棘层松解和角化相关。G1的结膜杯状细胞计数低于G3。在G2组犬的结膜样本中偶尔发现包涵体。G1和G2组眼部病变的严重程度与组织病理学结果无关。
非感染性和CDV诱导的KCS犬的结膜组织病理学非常相似。我们的研究结果表明，CDV诱导的KCS的病理生理学可能与非感染性KCS相同，即泪液缺乏和眼表面炎症的结果。
