Fullard R J, Kaswan R M, Bounous D I, Hirsh S G
School of Optometry, University of Alabama at Birmingham 35294-0010, USA.
J Am Optom Assoc. 1995 Jul;66(7):397-404.
Topically administered cyclosporine A (CsA) decreases ocular surface inflammation in canine keratoconjunctivitis sicca (KCS) and exerts lacrimomimetic effects. This study was performed to find correlations between clinical signs and tear protein levels in untreated and CsA-treated canine KCS.
Clinical profiles were scored in 16 KCS-affected dogs before and 6 weeks after commencing treatment with 0.2% topical CsA emulsion. Tear samples were also collected using polished micropipettes for specific protein assay by high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA).
Tear levels of serum proteins correlated with conjunctival clinical signs. Levels of lacrimal gland proteins in tears correlated most often with corneal clinical signs.
The inflammatory features of KCS appear to link conjunctival signs to serum proteins in tears, while corneal signs are linked to lacrimal gland proteins.
局部应用环孢素A（CsA）可减轻犬干眼症（KCS）的眼表炎症，并发挥拟泪作用。本研究旨在寻找未经治疗和经CsA治疗的犬KCS的临床体征与泪液蛋白水平之间的相关性。
对16只患KCS的犬在开始使用0.2%局部CsA乳剂治疗前和治疗6周后进行临床特征评分。还使用抛光微量移液器收集泪液样本，通过高效液相色谱（HPLC）和酶联免疫吸附测定（ELISA）进行特定蛋白质检测。
泪液中血清蛋白水平与结膜临床体征相关。泪液中泪腺蛋白水平最常与角膜临床体征相关。
KCS的炎症特征似乎将结膜体征与泪液中的血清蛋白联系起来，而角膜体征则与泪腺蛋白有关。
