Cafaro Thamara A, Ortiz Susana G, Maldonado Cristina, Espósito Fernando A, Croxatto Juan O, Berra Alejandro, Ale Omar L, Torrealday Juan I, Urrets-Zavalía Enrique A, Urrets-Zavalía Julio A, Serra Horacio M
Department of Clinical Biochemistry, Faculty of Chemical Science, National University of Córdoba, Córdoba, Argentina.
Vet Ophthalmol. 2009 Jul-Aug;12(4):234-41. doi: 10.1111/j.1463-5224.2009.00708.x.
To describe the functional and structural characteristics of the cornea in healthy Guinea pigs.
Healthy male and female pigmented and albino Guinea pigs (Caviaporcellus) aged 3-5 months old were used.
The animals' corneas underwent different in vivo studies including: slit-lamp biomicroscopy, fluorescein staining (FS), break-up time test (BUT), confocal microscopy and pachymetry. The corneas were also studied histopathologically with light microscopy, immunohistochemistry and transmission electron microscopy.
No significant differences were found between pigmented and albino animals, male and female, OD and OS in any study performed. The differences on corneal thickness values were not significant among central (227.85 +/- 14.09 microm) and upper and temporal peripheral regions (226.60 +/- 12.50 and 225.70 +/- 14.40 microm, respectively). All histological studies performed permitted identification and precise description of the different corneal structures in Guinea pigs: the stratified epithelium (45.52 +/- 5.26 microm), Bowman's layer (2.23 +/- 0.38 microm), stroma (163.69 +/- 4.90 microm), Descemet's membrane (3.96 +/- 0.46 microm) and the endothelium (5.09 +/- 0.71 microm). Combining results from all eyes mean and SD from corneal BUT values was 4.98 +/- 1.67 s. Corneas often showed discrete superficial erosions being the FS positive in both eyes from all the animals.
This study provides a detailed in vivo and postfixed histological description of the Guinea pig's cornea and information about the physiological tests.
描述健康豚鼠角膜的功能和结构特征。
使用3至5月龄的健康雄性和雌性有色及白化豚鼠（豚鼠）。
对动物的角膜进行了不同的体内研究，包括：裂隙灯生物显微镜检查、荧光素染色（FS）、泪膜破裂时间测试（BUT）、共聚焦显微镜检查和测厚法。还通过光学显微镜、免疫组织化学和透射电子显微镜对角膜进行了组织病理学研究。
在任何一项研究中，有色和白化动物、雄性和雌性、右眼和左眼之间均未发现显著差异。中央（227.85±14.09微米）与上方和颞侧周边区域（分别为226.60±12.50和225.70±14.40微米）的角膜厚度值差异不显著。所有组织学研究均能识别并精确描述豚鼠不同的角膜结构：分层上皮（45.52±5.26微米）、Bowman层（2.23±0.38微米）、基质（163.69±4.90微米）、Descemet膜（3.96±0.46微米）和内皮（5.09±0.71微米）。综合所有眼睛的结果，角膜BUT值的平均值和标准差为4.98±1.67秒。所有动物的双眼角膜常出现离散的浅表糜烂，FS检查呈阳性。
本研究提供了豚鼠角膜详细的体内和固定后组织学描述以及有关生理测试的信息。
