Hjortberg M, Jin M, Larsson A, Nilsson B O
Department of Human Anatomy, Uppsala University, Sweden.
J Reprod Immunol. 1991 Sep;20(3):277-87. doi: 10.1016/0165-0378(91)90052-r.
A Sepharose bead blot technique was used to study immunoglobulins in the uterine secretion of mice during the pre-implantation stage. Secretion collected by Sepharose beads contained IgA, IgG, and IgM. The method could be made ten-fold more sensitive by using anti-mouse IgG or IgM conjugated to Sepharose beads. It has also been demonstrated that when injected intravenously, biotinylated purified immunoglobulins, both non-specific mouse myeloma IgG and IgM and specific anti-blastocyst IgG and IgM, is able to pass into the uterine cavity of the mouse. It was further shown that when injected systemically, anti-blastocyst antibodies can reach the blastocyst. Functionally active specific antibodies against morulae and/or blastocysts may, therefore, be able to influence the pre- and peri-implantation development of the embryo and could serve as a useful model for experiments directed towards the identification of immunological contraceptive procedures.
采用琼脂糖珠印迹技术研究小鼠着床前子宫分泌物中的免疫球蛋白。用琼脂糖珠收集的分泌物含有IgA、IgG和IgM。通过使用与琼脂糖珠偶联的抗小鼠IgG或IgM，该方法的灵敏度可提高10倍。还证明，静脉注射生物素化的纯化免疫球蛋白，无论是非特异性小鼠骨髓瘤IgG和IgM，还是特异性抗胚泡IgG和IgM，都能够进入小鼠子宫腔。进一步表明，全身注射抗胚泡抗体可到达胚泡。因此，针对桑椹胚和/或胚泡的具有功能活性的特异性抗体可能会影响胚胎着床前和着床期的发育，并可作为鉴定免疫避孕方法实验的有用模型。
