Hjortberg M, Pihl M, Grönvik K O, Nilsson B O
Department of Human Anatomy, Uppsala University, Sweden.
J Reprod Immunol. 1993 Jan;23(1):83-92. doi: 10.1016/0165-0378(93)90029-h.
Five monoclonal anti-mouse-blastocyst IgG antibodies were raised by intrasplenic immunization of three mice with adhesive-stage mouse blastocysts. Each mouse received a total of 60-70 blastocysts which were either nitrocellulose-immobilized or living but irradiated. Tests for pre-implantation stage-specificity showed that the antibodies differed in specificity. None were specific for surface epitopes. One antibody recognized epitopes only on blastocysts. Other antibodies were able to discriminate between unfertilized and fertilized oocytes, uncompacted and compacted morulae, or delayed and adhesive blastocysts. By applying reduced SDS-PAGE and Western blotting to blastocysts the blastocyst-specific antibody was seen to be bound to a peptide of M(r) 34.
通过用黏附期小鼠囊胚对三只小鼠进行脾内免疫，产生了五种单克隆抗小鼠囊胚IgG抗体。每只小鼠共接受60 - 70个囊胚，这些囊胚要么是固定在硝酸纤维素上的，要么是活的但经过辐照的。着床前阶段特异性测试表明，这些抗体的特异性有所不同。没有一种对表面表位具有特异性。一种抗体仅识别囊胚上的表位。其他抗体能够区分未受精卵母细胞和受精卵母细胞、未致密化和致密化桑椹胚，或延迟和黏附的囊胚。通过对囊胚应用还原SDS - 聚丙烯酰胺凝胶电泳和蛋白质印迹法，发现囊胚特异性抗体与分子量为34的肽结合。
