Crowley Tamsyn M, Haring Volker R, Burggraaf Simon, Moore Robert J
Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia.
BMC Genomics. 2009 Jul 14;10 Suppl 2(Suppl 2):S3. doi: 10.1186/1471-2164-10-S2-S3.
With the threat of emerging infectious diseases such as avian influenza, whose natural hosts are thought to be a variety of wild water birds including duck, we are armed with very few genomic resources to investigate large scale immunological gene expression studies in avian species. Multiple options exist for conducting large gene expression studies in chickens and in this study we explore the feasibility of using one of these tools to investigate gene expression in other avian species.
In this study we utilised a whole genome long oligonucleotide chicken microarray to assess the utility of cross species hybridisation (CSH). We successfully hybridised a number of different avian species to this array, obtaining reliable signals. We were able to distinguish ducks that were infected with avian influenza from uninfected ducks using this microarray platform. In addition, we were able to detect known chicken immunological genes in all of the hybridised avian species.
Cross species hybridisation using long oligonucleotide microarrays is a powerful tool to study the immune response in avian species with little available genomic information. The present study validated the use of the whole genome long oligonucleotide chicken microarray to investigate gene expression in a range of avian species.
随着诸如禽流感等新发传染病的威胁，其天然宿主被认为是包括鸭在内的多种野生水鸟，我们用于开展鸟类大规模免疫基因表达研究的基因组资源非常有限。在鸡中进行大规模基因表达研究有多种选择，在本研究中，我们探索使用其中一种工具来研究其他鸟类基因表达的可行性。
在本研究中，我们利用全基因组长寡核苷酸鸡微阵列来评估跨物种杂交（CSH）的效用。我们成功地将多种不同的鸟类与该阵列进行杂交，获得了可靠的信号。利用这个微阵列平台，我们能够区分感染禽流感的鸭和未感染的鸭。此外，我们能够在所有杂交的鸟类物种中检测到已知的鸡免疫基因。
使用长寡核苷酸微阵列进行跨物种杂交是研究基因组信息有限的鸟类免疫反应的有力工具。本研究验证了使用全基因组长寡核苷酸鸡微阵列来研究一系列鸟类物种中的基因表达。
